Active Recombinant Human DEFB4 protein(1-50aa)

• Cat.No.: DEFB4-18H
• Product Overview: Recombinant Human DEFB4 protein(Q8WTQ1)(50aa) was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: N/A
• Protein length: 1-50aa
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.
• Molecular Mass: Approximately 6.0 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.
• AA Sequence: EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP
• Endotoxin: Less than 1 EU/μg of rHuBD-4 as determined by LAL method.
• Purity: > 98 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  - 12 months from date of receipt, -20 to -70 °C as supplied.
  - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: DEFB104A defensin beta 104A [ Homo sapiens (human) ]
• Official Symbol: DEFB104A
• Synonyms: BD-4; DEFB4; hBD-4; DEFB-4; DEFB104
• Gene ID: 140596
• mRNA Refseq: NM_080389
• Protein Refseq: NP_525128
• UniProt ID: Q496I2