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Recombinant Human Chemokine (C-C Motif) Ligand 1

Cat. No. : CCL1-29H
Product Overview : Recombinant Human I-309 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8504 Dalton. The I-309 is purified by proprietary chromatographic techniques.
Description : Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. This chemokine resides in a large cluster of CC chemokines on human chromosome 17.
Source : Escherichia Coli.
Amino Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Ser-Met-Gln.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Purity : Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : The protein was lyophilized with no additives.
Solubility : It is recommended to reconstitute the lyophilized I-309 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity : The Biological activity is calculated by its ability to chemoattract human T cells at 10.0-100.0 ng/ml.
Storage : Lyophilized I-309 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL1 should be stored at 4℃between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name : CCL1 chemokine (C-C motif) ligand 1 [ Homo sapiens ]
Synonyms : CCL1; chemokine (C-C motif) ligand 1; C-C motif chemokine 1; I-309; inflammatory cytokine I-309; P500; SISe; SCYA1; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); Small-inducible cytokine A1; T lymphocyte-secreted protein I-309; TCA3
Gene ID : 6346
mRNA Refseq : NM_002981
Protein Refseq : NP_002972
MIM : 182281
UniProt ID : P22362
Chromosome Location : 17q12
Pathway : Chemokine signaling pathway; Cytokine-cytokine receptor interaction
Function : chemokine activity; protein binding

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