||Recombinant Human CD-4 is expressed in E. coli encoding 125-202 amino acids, having a total molecular weight of 15kDa and fused to an amino terminal hexahistidine tag.
||CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E-cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.
||Sterile lyophilized powder.
||Greater than 90% determined by SDS-PAGE.
||Each mg contains 1X PBS.
||It is recommended to reconstitute the lyophilized CD-4 (125-202) in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||• Protein Assay • Antibody Production • Western Blotting
||Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12months. Please avoid freeze-thaw cycles.