| Description : |
Human Recombinant Epidermal Growth Factor, (rh EGF) is a is a small mitogenic polypeptide which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids. EGF stimulates the proliferation and differentiation of epithelial cells from skin, cornea, lung and tracheal tissue and the gastrointestinal tract. EGF also promotes growth and migration of keratinocytes and enhances the proliferation of fibroblasts and embryonic cells. It is a member of a growth factor family which is characterized by the presence of 6 conserved cysteine motifs that form three disulphide bonds. The biological effects of EGF are mediated by a specific transmembrane receptor (EGF-R). The binding of EGF to EGFR will induce receptor dimerization, which is required for activating the tyrosine kinase in the receptor cytoplasmic domain. Thus, EGF triggers several signal transduction pathways including JAK/STAT, Ras/ERK and PI3K/AKT pathways.EGF plays an important role in wound healing and organogenesis .In addition to its proliferative effects, it participle of a variety of other bioactivities, including effects on cytoskeletal organization, cell migration and the synthesis and turnover of extracellular matrix molecules. Therefore, hEGF has wide application prospects in clinical and cosmetic fields. |
| Source : |
Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal–derived components or impurities. Animal Free product. |
| Species : |
Human |
| Tag : |
His |
| Form : |
Recombinant human EGF is lyophilized from 10mM Phosphate Potasium buffer pH 7.5 and 230 mM NaCl. |
| Molecular Mass : |
7 kDa |
| AA Sequence : |
HHHHHHNSDSECPLSHDGYCLHDGV CMYIEALDKYACNCVVGYIGERCQY RDLKWWELR |
| Endotoxin : |
< 0.04 eu/μg protein (lal> |
| Purity : |
>97% by SDS-PAGE gel |
| Storage : |
This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted protein should be stored in working aliquots at –20°C. Repeated freezing and thawing is not recommended. |
| Reconstitution : |
Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 200 ng/μl. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. Optimal concentration should be determined for specific application and cell lines. |
