Recombinant Mouse TIGIT cell lysate
Cat.No. : | TIGIT-1420MCL |
Product Overview : | Mouse TIGIT / VSTM3 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Tigit T cell immunoreceptor with Ig and ITIM domains [ Mus musculus ] |
Official Symbol : | TIGIT |
Synonyms : | TIGIT; T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Vstm3; ENSMUSG00000071552; |
Gene ID : | 100043314 |
mRNA Refseq : | NM_001146325 |
Protein Refseq : | NP_001139797 |
Function : | molecular_function; |
Products Types
◆ Recombinant Protein | ||
TIGIT-306H | Recombinant Human TIGIT Protein (ECD), His-tagged(C-ter) | +Inquiry |
TIGIT-12H | Recombinant Human TIGIT Protein, C-6His-Avi tagged, Biotinylated | +Inquiry |
TIGIT-2193H | Recombinant Human TIGIT Protein, His (Fc)-Avi-tagged | +Inquiry |
TIGIT-733R | Recombinant Rat TIGIT Protein, Fc-tagged | +Inquiry |
TIGIT-356H | Active Recombinant Human TIGIT Protein (Met22-Pro141, A103), C-Fc-tagged | +Inquiry |
◆ Lysates | ||
TIGIT-2610HCL | Recombinant Human TIGIT cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionTIGIT inhibitors are being studied in a range of cancers, including lung cancer, breast cancer, and melanoma, among others.
TIGIT is being explored as a potential target for treating autoimmune diseases by modulating immune responses to prevent excessive inflammation.
TIGIT is being explored as a potential target for cancer immunotherapy, with drugs designed to block TIGIT and enhance the immune system's ability to attack cancer cells.
TIGIT operates as an immune checkpoint, similar to PD-1 and CTLA-4, but it has distinct mechanisms of action and may regulate different aspects of the immune response.
As with any immunotherapy, potential side effects may include immune-related adverse events such as inflammation in various organs, but the specific profile is still under investigation.
Customer Reviews (3)
Write a reviewWith its well-characterized nature and availability in various formats, the TIGIT protein is an advantageous tool that aids researchers in achieving their scientific goals.
Whether it's troubleshooting issues, optimizing experimental protocols, or providing detailed information about the protein, the manufacturer's support team is dedicated to helping researchers overcome any challenges they may encounter.
The manufacturer also ensures that the TIGIT protein is well-characterized, with extensive documentation on its functional properties.
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