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Recombinant Human CLEC10A cell lysate

Cat.No. : CLEC10A-1456HCL
Product Overview : Human CLEC10A / MGL1 / CD301 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CLEC10A C-type lectin domain family 10, member A [ Homo sapiens ]
Official Symbol : CLEC10A
Synonyms : CLEC10A; C-type lectin domain family 10, member A; C type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 14 (macrophage derived) , CLECSF13, CLECSF14; C-type lectin domain family 10 member A; CD301; HML; HML2; macrophage lectin 2 (calcium dependent); C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13 (macrophage-derived); C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 14 (macrophage-derived); MGL; CLECSF13; CLECSF14;
Gene ID : 10462
mRNA Refseq : NM_006344
Protein Refseq : NP_006335
MIM : 605999
UniProt ID : Q8IUN9
Chromosome Location : 17p13.2
Function : binding; sugar binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can CLEC10A protein be targeted for immunotherapy? 03/16/2020

Researchers are exploring the potential of targeting CLEC10A to modulate immune responses against cancer and infectious diseases, with the aim of developing novel immunotherapies.

How does CLEC10A protein interact with the immune system? 03/20/2019

CLEC10A functions as a receptor on immune cells, where it can recognize specific glycan structures on pathogens and tumor cells, leading to immune cell activation and modulation of immune responses.

What are the potential clinical implications of modulating CLEC10A activity? 12/16/2018

Modulating CLEC10A activity could have therapeutic implications for cancer immunotherapy, infectious disease treatment, and potentially other immune-related disorders.

How does CLEC10A protein impact the tumor microenvironment? 07/15/2018

CLEC10A expression on tumor-associated macrophages and dendritic cells can influence the immune response within the tumor microenvironment, potentially impacting tumor growth and metastasis.

Is CLEC10A protein expressed in specific cell types? 02/21/2018

CLEC10A is primarily expressed on dendritic cells and macrophages in the immune system, where it plays a role in antigen presentation and immune regulation.

Customer Reviews (3)

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Reviews
04/30/2022

    The CLEC10A protein exhibits remarkable performance in Western blotting (WB) experiments.

    01/07/2020

      The versatility and efficacy of CLEC10A in these applications make it an invaluable tool for researchers.

      09/17/2018

        Researchers can rely on CLEC10A to deliver reliable and accurate results in ELISA experiments, ensuring precise measurement and quantification of target proteins.

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