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Recombinant Human PLA2G4A 293 Cell Lysate

Cat.No. : PLA2G4A-3141HCL
  • Specification
  • Gene Information
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Description : Antigen standard for phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PLA2G4A phospholipase A2, group IVA (cytosolic, calcium-dependent) [ Homo sapiens ]
Official Symbol : PLA2G4A
Synonyms : PLA2G4A; phospholipase A2, group IVA (cytosolic, calcium-dependent); PLA2G4; cytosolic phospholipase A2; cPLA2 alpha; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein; cPLA2-alpha; MGC126350;
Gene ID : 5321
mRNA Refseq : NM_024420
Protein Refseq : NP_077734
UniProt ID : P47712
Chromosome Location : 1q25
Pathway : ADP signalling through P2Y purinoceptor 1, organism-specific biosystem; Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Ca-dependent events, organism-specific biosystem; Endothelins, organism-specific biosystem; Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem;
Function : calcium ion binding; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; hydrolase activity; lysophospholipase activity; phospholipase A2 activity; phospholipase A2 activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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