"MAPK10" Related Products

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Recombinant Human MAPK10 293 Cell Lysate

Cat.No.: MAPK10-4499HCL
Description: Antigen standard for mitogen-activated protein kinase 10 (MAPK10), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MAPK10 mitogen-activated protein kinase 10 [ Homo sapiens ]
Official Symbol: MAPK10
Synonyms: MAPK10; mitogen-activated protein kinase 10; PRKM10; JNK3; p54bSAPK; p493F12; MAPK 10; MAP kinase 10; MAP kinase p49 3F12; JNK3 alpha protein kinase; c-Jun N-terminal kinase 3; stress-activated protein kinase 1b; stress activated protein kinase beta; stress-activated protein kinase JNK3; JNK3A; SAPK1b; FLJ12099; FLJ33785; MGC50974;
Gene ID: 5602
mRNA Refseq: NM_002753
Protein Refseq: NP_002744
MIM: 602897
UniProt ID: P53779
Chromosome Location: 4q22-q23
Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of the AP-1 family of transcription factors, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis Modulation by HSP70, organism-specific biosystem; CD40/CD40L signaling, organism-specific biosystem;
Function: ATP binding; JUN kinase activity; MAP kinase activity; MAP kinase kinase activity; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups;

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