Recombinant Human Factor XII

• Cat.No.: F12 -82H
• Product Overview: Recombinant Human Factor XII is a single chain glycoprotein produced in insect cell culture. Factor XII is converted, principally from the action of kallikrein, into an active serine protease (Factor alpha-Xlla) that functions in the in vivo initiation of blood coagulation, fibrinolysis, and kinin formation. The protein purity is determined by SDS-PAGE and activity is determined via complementation in an APTT clotting assay using Factor XII immuno-deficient human plasma. Specific activity of this recombinant product is typically lower than that of plasma derived material. Custom mutants of Factor XII are also available, please contact us for more details.

Specification

• Source: Insect cell
• Species: Human
• Form: Frozen liquid
• Molecular Mass: 80,000 (Human Plasma Derived)
• Purity: >95% by SDS-PAGE analysis
• Usage: For in vitro laboratory use only
• Notes: Optimal experimental conditions and working dilutions must be determined by the end user. Sample concentration and volume are for example purposes only and do not necessarily represent the product that will be received. Please contact us for a current lot-specific product datasheet.
• Storage: -70°C
• Concentration: 0.25 mg/ml
• Storage Buffer: 4 mM Sodium Acetate; 0.15 M NaCl; pH 5.3
• Shipping: Dry ice

Gene Information

• Gene Name: F12 coagulation factor XII (Hageman factor) [ Homo sapiens ]
• Official Symbol: F12
• Synonyms: F12; coagulation factor XII (Hageman factor); coagulation factor XII; Hageman factor; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain; HAF; HAE3; HAEX
• Gene ID: 2161
• mRNA Refseq: NM_000505
• Protein Refseq: NP_000496
• MIM: 610619
• UniProt ID: P00748
• Chromosome Location: 5q33-qter
• Pathway: Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Hemostasis, organism-specific biosystem; Intrinsic Pathway, organism-specific biosystem
• Function: binding; misfolded protein binding; peptidase activity; protein binding; serine-type endopeptidase activity