Active Recombinant Mouse Il3

• Cat.No.: Il3-644M
• Product Overview: Recombinant Mouse Il3 (Accession # NP_034686) Asp33-Cys166, fused with an N-terminal Met, was produced in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Predicted N Terminal: Asp33
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. Holmes, K.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:6687.The ED50 for this effect is typically 20-100 pg/mL.
• Molecular Mass: Recombinant Mouse Il3, Met-tagged has a calculated MW of 15 kDa.
• Protein length: Asp33-Cys166
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Il3 interleukin 3 [ Mus musculus ]
• Official Symbol: Il3
• Synonyms: IL3; interleukin 3; interleukin-3; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor; BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• Gene ID: 16187
• mRNA Refseq: NM_010556
• Protein Refseq: NP_034686