Recombinant Mouse Nphs1 Protein, His-tagged Nphs1-718M

Recombinant Mouse Nphs1 Protein, His-tagged


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Recombinant Mouse Nphs1 Protein, His-tagged

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Cat.No. : Nphs1-718M
Product Overview : Recombinant Mouse Nphs1 (Accession # NP_062332) Gln37-Thr1049, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Source : NSO Cells
Species : Mouse
Tag : His
Predicted N Terminal : No results obtained: Gln37 predicted
Form : Lyophilized from a 0.2 µm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of MS-1 mouse pancreatic islet endothelial cells.When 5 x 10^4 cells/well are added to rmNephrin coated plates (10 µg/mL, 100 µL/well), approximately 60 - 80% will adhere after 90 minutes at 37° C.
Molecular Mass : Recombinant Mouse Nphs1, His-tagged has a calculated MW of 110 kDa. In SDS-PAGE migrates as 130-145 kDa, reducing conditions.
Protein length : Gln37-Thr1049
Purity : >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Nphs1 nephrosis 1 homolog, nephrin (human) [ Mus musculus ]
Official Symbol : Nphs1
Synonyms : NPHS1; nephrosis 1 homolog, nephrin (human); nephrin; nephrin 1; renal glomerulus-specific cell adhesion receptor; NephrinB;
Gene ID : 54631
mRNA Refseq : NM_019459
Protein Refseq : NP_062332

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