Active Recombinant Mouse Nphs1 Protein, His-tagged

• Cat.No.: Nphs1-718M
• Product Overview: Recombinant Mouse Nphs1 (Accession # NP_062332) Gln37-Thr1049, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: No results obtained: Gln37 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MS-1 mouse pancreatic islet endothelial cells.When 5 x 10^4 cells/well are added to rmNephrin coated plates (10 µg/mL, 100 µL/well), approximately 60 - 80% will adhere after 90 minutes at 37° C.
• Molecular Mass: Recombinant Mouse Nphs1, His-tagged has a calculated MW of 110 kDa. In SDS-PAGE migrates as 130-145 kDa, reducing conditions.
• Protein length: Gln37-Thr1049
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Nphs1 nephrosis 1 homolog, nephrin (human) [ Mus musculus ]
• Official Symbol: Nphs1
• Synonyms: NPHS1; nephrosis 1 homolog, nephrin (human); nephrin; nephrin 1; renal glomerulus-specific cell adhesion receptor; NephrinB
• Gene ID: 54631
• mRNA Refseq: NM_019459
• Protein Refseq: NP_062332