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Recombinant Human KIT Ligand, His-tagged

Cat. No.: KITLG-60H
Product Overview: Recombinant Human Stem Cell Factor produced ininsect cellsis a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is fused to a C-terminal His-tag and purified by proprietary chromatographic techniques.
Description: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117 (c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
Source: Insect Cells.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: The protein is supplied in 1X PBS, pH 7.4.
Solubility: It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The ED50as determined by the dose-dependant stimulation of Human TF-1 cells is typically 2-5 ng/ml.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.
Storage: Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution SCF should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: KITLG KIT ligand [ Homo sapiens ]
Synonyms: KITLG; KIT ligand; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7; DKFZp686F2250; KITLG; C-kit ligand; Stem cell factor; Mast cell growth factor;steel factor
Gene ID: 4254
mRNA Refseq: NM_000899
Protein Refseq: NP_000890
UniProt ID: P21583
Chromosome Location: 12q22
MIM: 184745
Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Melanogenesis; Pathways in cancer
Function: growth factor activity; identical protein binding; stem cell factor receptor binding

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