Recombinant Human FGF9 protein, Fc-tagged
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|Product Overview :||Recombinant Human FGF9 (Leu4-Ser208) protein was fused to human IgG1 Fc tag at N-terminus and expressed in human 293 cells (HEK293).|
|Description :||Fibroblast growth factor 9 is also known as FGF9, GAF, HBFG-9, SYNS3, and is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 is also a mitogen for oligodendrocyte type 2 astrocyte progenitor cells, smooth muscle cells, pheochromocytoma PC12 cells, and BALB/3T3 fibroblasts. However, unlike FGF acidic and basic, FGF9 has no effect on human umbilical vein endothelial cells, and it has been demonstrated that FGF9 binds preferentially to the IIIc form of FGFR3. Although no typical signal sequence was found in FGF9, it is secreted efficiently after synthesis not in a conventional manner. In nervous system, FGF9 is produced mainly by neurons and may plays an important role in CNS development. FGF9 has been shown to interact with Fibroblast growth factor receptor 3.|
|Tag :||human IgG1 Fc|
|Predicted N Terminal :||Leu4|
|Form :||Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 50.6 kDa. As a result of glycosylation, the protein migrates as 55 kDa under reducing (R) condition, and 110 kDa under non-reducing (NR) condition (SDS-PAGE).|
|Protein length :||Leu4-Ser208|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>92% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of FGF9-202H :||
Human FGF-9, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
|Gene Name :||FGF9|
|Official Symbol :||FGF9|
|Synonyms :||FGF9; fibroblast growth factor 9 (glia-activating factor); fibroblast growth factor 9; FGF-9; HBGF-9; heparin-binding growth factor 9; GAF; SYNS3; HBFG-9; MGC119914; MGC119915|
|Gene ID :||2254|
|mRNA Refseq :||NM_002010|
|Protein Refseq :||NP_002001|
|UniProt ID :||P31371|
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