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Recombinant Human Growth Differentiation Factor 9, His-tagged

Cat. No.: GDF9-223H
Product Overview: Recombinant Human Growth Differentiation Factor 9 encoding the mature human Growth differentiation factor 9 (Gln320-Arg454P) was recombined with his tag at the N-Terminus. That was expressed inE.Coli, and purified by Ni-NTA column. MW=14.9KD.
Description: Growth/differentiation factor 9 is a protein that in humans is encoded by the GDF9 gene. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. Growth differentiation factor-9 (GDF9) is expressed in oocytes and is thought to be required for ovarian folliculogenesis. GDF9 is a member of the transforming growth factor-beta (TGFβ) superfamily. GDF9 plays an important role in the development of primary follicles in the ovary. It has a critical role in granulosa cell and theca cell growth, as well as in differentiation and maturation of the oocyte.
Source: E. Coli.
Sequence: Human Growth differentiation factor (Gln320-Arg454P)320GQETVSSELK KPLGPASFNL SEYFRQFLLP QNECELHDFR LSFSQLKWDN WIVAPHRYNP RYCKGDCPRA VGHRYGSPVH TMVQNIIYEK LDSSVPRPSC VPAKYSPLSV LTIEPDGSIA YKEYEDMIAT KCTCR 454.
Purity: >95% in SDS gel.
Formulation: Lyophilized 100 μg human Growth differentiation factor 9 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
Application: Cell biology, ELISA.
Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
Storage: Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
Gene Name: GDF9 growth differentiation factor 9 [ Homo sapiens ]
Synonyms: GDF9; growth differentiation factor 9; OTTHUMP00000066213;GDF-9; Growth/differentiation factor 9
Gene ID: 2661
mRNA Refseq: NM_005260
Protein Refseq: NP_005251
MIM: 601918
UniProt ID: O60383
Chromosome Location: 5q31.1
Function: cytokine activity; growth factor activity

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