| Species : |
Mouse |
| Source : |
HEK293 |
| Tag : |
Flag |
| Protein Length : |
115-260 a.a. |
| Description : |
CD154, also called CD40 ligand or CD40L, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40L plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome. |
| Purity : |
≥90% (SDS-PAGE). |
| Concentration : |
0.1mg/ml after reconstitution. |
| Formulation : |
Lyophilized. Contains PBS. |
| Endotoxin Content : |
<0.1EU/µg purified protein (LAL test; Bio Whittaker). |
| Reconstitution : |
Reconstitute each tube with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
| Specificity : |
Binds to human and mouse CD40. |
| Biological Activity : |
Stimulates the proliferation of mouse B cells and dendritic cells. The activity of rmsCD40L increases 1"000-fold (stimulation in the ng/ml range) in the presence of a cross-linking enhancer. |
| Storage : |
-20°C. |
