Recombinant Human ROCK2, GST-His

• Cat.No.: ROCK2-712H
• Product Overview: Recombinant Human ROCK2, Amino acids P6-S553, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 92,279 Da. .

Specification

• Cat. No.: ROCK2-712H
• Description: ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. ROCK2 is an immediate downstream target of the small GTPase RhoA.
• Source: Baculovirus infected Sf9 cells.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: ROCK2 was confirmed as human ROCK2 by mass spec troscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.356 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 27 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: ROCK2 Rho-associated, coiled-coil containing protein kinase 2 [ Homo sapiens ]
• Synonyms: ROCK2; Rho-associated, coiled-coil containing protein kinase 2; KIAA0619; EC 2.7.11.1
• Gene ID: 9475
• mRNA Refseq: NM_004850
• Protein Refseq: NP_004841
• MIM: 604002
• UniProt ID: O75116
• Chromosome Location: 2p24
• Pathway: Axon guidance; Chemokine signaling pathway; Focal adhesion; Leukocyte transendothelial migration; Pathogenic Escherichia coli infection; Regulation of actin cytoskeleton; TGF-beta signaling pathway; Vascular smooth muscle contraction; Wnt signaling pathway; Signaling by GPCR; Axon guidance
• Function: ATP binding; GTP-Rho binding; identical protein binding; magnesium ion binding; nucleotide binding; protein serine/threonine kinase activity; zinc ion binding; transferase activity