| Cat.No. : |
CLPP-1971C |
| Product Overview : |
Recombinant Clostridium Botulinum (strain Hall/ATCC 3502/NCTC 13319/Type A) CLPP Protein (1-194 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Microbiology. Protein Description: Full Length. |
| Description : |
Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins. Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). |
| Source : |
E. coli |
| Species : |
Clostridium Botulinum |
| Tag : |
His-SUMO |
| Form : |
Tris-based buffer,50% glycerol |
| Molecular Mass : |
37.5 kDa |
| Protein length : |
1-194 aa |
| AA Sequence : |
MSLVPVVVEQ TNRGERSYDI YSRLLKDRII MLSEEVNDTT ASLIVAQLLF LEAEDPDKDI HLYINSPGGS ITSGMAIYDT MQYIKPDVST ICVGMAASMG AFLLAAGAKG KRYALPNSEV MIHQPLGGFR GQATDIGIHA ERILKMKKKL NTILSDRTGK PLEQVELDTE RDHFLSAEEA KEYGLIDEVI DKKK |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : |
A hardcopy of COA with reconstitution instruction is sent along with the products. |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.