Recombinant Full Length Human A2M Protein, C-Flag-tagged

• Cat.No.: A2M-1097HFL
• Product Overview: Recombinant Full Length Human A2M Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 160.8 kDa
• AA Sequence: MGKNKLLHPSLVLLLLVLLPTDASV SGKPQYMVLVPSLLHTETTEKGCVL LSYLNETVTVSASLESVRGN RSLFTDLEAENDVLHCVAFAVPKSS SNEEVMFLTVQVKGPTQEFKKRTTV MVKNEDSLVFVQTDKSIYKP GQTVKFRVVSMDENFHPLNELIPLV YIQDPKGNRIAQWQSFQLEGGLKQF SFPLSSEPFQGSYKVVVQKK SGGRTEHPFTVEEFVLPKFEVQVTV PKIITILEEEMNVSVCGLYTYGKPV PGHVTVSICRKYSDASDCHG EDSQAFCEKFSGQLNSHGCFYQQVK TKVFQLKRKEYEMKLHTEAQIQEEG TVVELTGRQSSEITRTITKL SFVKVDSHFRQGIPFFGQVRLVDGK GVPIPNKVIFIRGNEANYYSNATTD EHGLVQFSINTTNVMGTSLT VRVNYKDRSPCYGYQWVSEEHEEAH HTAYLVFSPSKSFVHLEPMSHELPC GHTQTVQAHYILNGGTLLGL KKLSFYYLIMAKGGIVRTGTHGLLV KQEDMKGHFSISIPVKSDIAPVARL LIYAVLPTGDVIGDSAKYDV ENCLANKVDLSFSPSQSLPASHAHL RVTAAPQSVCALRAVDQSVLLMKPD AELSASSVYNLLPEKDLTGF PGPLNDQDDEDCINRHNVYINGITY TPVSSTNEKDMYSFLEDMGLKAFTN SKIRKPKMCPQLQQYEMHGP EGLRVGFYESDVMGRGHARLVHVEE PHTETVRKYFPETWIWDLVVVNSAG VAEVGVTVPDTITEWKAGAF CLSEDAGLGISSTASLRAFQPFFVE LTMPYSVIRGEAFTLKATVLNYLPK CIRVSVQLEASPAFLAVPVE KEQAPHCICANGRQTVSWAVTPKSL GNVNFTVSAEALESQELCGTEVPSV PEHGRKDTVIKPLLVEPEGL EKETTFNSLLCPSGGEVSEELSLKL PPNVVEESARASVSVLGDILGSAMQ NTQNLLQMPYGCGEQNMVLF APNIYVLDYLNETQQLTPEVKSKAI GYLNTGYQRQLNYKHYDGSYSTFGE RYGRNQGNTWLTAFVLKTFA QARAYIFIDEAHITQALIWLSQRQK DNGCFRSSGSLLNNAIKGGVEDEVT LSAYITIALLEIPLTVTHPV VRNALFCLESAWKTAQEGDHGSHVY TKALLAYAFALAGNQDKRKEVLKSL NEEAVKKDNSVHWERPQKPK APVGHFYEPQAPSAEVEMTSYVLLA YLTAQPAPTSEDLTSATNIVKWITK QQNAQGGFSSTQDTVVALHA LSKYGAATFTRTGKAAQVTIQSSGT FSSKFQVDNNNRLLLQQVSLPELPG EYSMKVTGEGCVYLQTSLKY NILPEKEEFPFALGVQTLPQTCDEP KAHTSFQISLSVSYTGSRSASNMAI VDVKMVSGFIPLKPTVKMLE RSNHVSRTEVSSNHVLIYLDKVSNQ TLSLFFTVLQDVPVRDLKPAIVKVY DYYETDEFAIAEYNAPCSKD
  LGNATRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades

Gene Information

• Gene Name: A2M alpha-2-macroglobulin [ Homo sapiens (human) ]
• Official Symbol: A2M
• Synonyms: A2MD; CPAMD5; FWP007; S863-7
• Gene ID: 2
• mRNA Refseq: NM_000014.6
• Protein Refseq: NP_000005.3
• MIM: 103950
• UniProt ID: P01023