Recombinant Full Length Human A2M Protein, C-Flag-tagged

• Cat.No.: A2M-1097HFL
• Product Overview: Recombinant Full Length Human A2M Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 160.8 kDa
• AA Sequence: MGKNKLLHPSLVLLLLVLLPTDASV SGKPQYMVLVPSLLHTETTEKGCVL LSYLNETVTVSASLESVRGN RSLFTDLEAENDVLHCVAFAVPKSS SNEEVMFLTVQVKGPTQEFKKRTTV MVKNEDSLVFVQTDKSIYKP GQTVKFRVVSMDENFHPLNELIPLV YIQDPKGNRIAQWQSFQLEGGLKQF SFPLSSEPFQGSYKVVVQKK SGGRTEHPFTVEEFVLPKFEVQVTV PKIITILEEEMNVSVCGLYTYGKPV PGHVTVSICRKYSDASDCHG EDSQAFCEKFSGQLNSHGCFYQQVK TKVFQLKRKEYEMKLHTEAQIQEEG TVVELTGRQSSEITRTITKL SFVKVDSHFRQGIPFFGQVRLVDGK GVPIPNKVIFIRGNEANYYSNATTD EHGLVQFSINTTNVMGTSLT VRVNYKDRSPCYGYQWVSEEHEEAH HTAYLVFSPSKSFVHLEPMSHELPC GHTQTVQAHYILNGGTLLGL KKLSFYYLIMAKGGIVRTGTHGLLV KQEDMKGHFSISIPVKSDIAPVARL LIYAVLPTGDVIGDSAKYDV ENCLANKVDLSFSPSQSLPASHAHL RVTAAPQSVCALRAVDQSVLLMKPD AELSASSVYNLLPEKDLTGF PGPLNDQDDEDCINRHNVYINGITY TPVSSTNEKDMYSFLEDMGLKAFTN SKIRKPKMCPQLQQYEMHGP EGLRVGFYESDVMGRGHARLVHVEE PHTETVRKYFPETWIWDLVVVNSAG VAEVGVTVPDTITEWKAGAF CLSEDAGLGISSTASLRAFQPFFVE LTMPYSVIRGEAFTLKATVLNYLPK CIRVSVQLEASPAFLAVPVE KEQAPHCICANGRQTVSWAVTPKSL GNVNFTVSAEALESQELCGTEVPSV PEHGRKDTVIKPLLVEPEGL EKETTFNSLLCPSGGEVSEELSLKL PPNVVEESARASVSVLGDILGSAMQ NTQNLLQMPYGCGEQNMVLF APNIYVLDYLNETQQLTPEVKSKAI GYLNTGYQRQLNYKHYDGSYSTFGE RYGRNQGNTWLTAFVLKTFA QARAYIFIDEAHITQALIWLSQRQK DNGCFRSSGSLLNNAIKGGVEDEVT LSAYITIALLEIPLTVTHPV VRNALFCLESAWKTAQEGDHGSHVY TKALLAYAFALAGNQDKRKEVLKSL NEEAVKKDNSVHWERPQKPK APVGHFYEPQAPSAEVEMTSYVLLA YLTAQPAPTSEDLTSATNIVKWITK QQNAQGGFSSTQDTVVALHA LSKYGAATFTRTGKAAQVTIQSSGT FSSKFQVDNNNRLLLQQVSLPELPG EYSMKVTGEGCVYLQTSLKY NILPEKEEFPFALGVQTLPQTCDEP KAHTSFQISLSVSYTGSRSASNMAI VDVKMVSGFIPLKPTVKMLE RSNHVSRTEVSSNHVLIYLDKVSNQ TLSLFFTVLQDVPVRDLKPAIVKVY DYYETDEFAIAEYNAPCSKD
  LGNATRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades

Gene Information

• Gene Name: A2M alpha-2-macroglobulin [ Homo sapiens (human) ]
• Official Symbol: A2M
• Synonyms: A2MD; CPAMD5; FWP007; S863-7
• Gene ID: 2
• mRNA Refseq: NM_000014.6
• Protein Refseq: NP_000005.3
• MIM: 103950
• UniProt ID: P01023

Reviews

04/19/2022

It can be seen that the production process of A2M pays great attention to purity and biological activity, which makes its products have extremely high application value.

05/23/2020

Short half-life and high clearance of A2M make them more suitable for use in emergency medical situations and provide more treatment options for patients.

03/09/2020

The production process of A2M achieves efficient use of energy, reduces energy consumption, reduces carbon emissions, and is very environmentally friendly.

Q&As

What role does A2M protein play in the immune system?

It play an important role in the immune system, binding and neutralizing viruses, bacteria, reducing inflammation, and participating in immune regulation.

What is the relationship between A2M protein and lung disease?

Studies of the A2M protein in lung disease have shown that it may work by inhibiting inflammation and reducing lung tissue damage.

In which organs or tissues are A2M proteins expressed high?

The expression of A2M protein in organs or tissues such as liver, kidney, and lymphoid tissue is high.

How does A2M protein remove harmful proteins from the body?

Proteins eventually clear harmful proteins by capturing and binding to harmful proteins and then entering the cell through internalization.

Can A2M protein be used as a potential therapeutic target for cardiovascular disease?

A2M proteins show potential therapeutic targets in cardiovascular diseases and may have effects on cardiovascular diseases by modulating inflammatory responses and blood clotting.

In which neurological diseases are A2M proteins studied?

A2M proteins are studied in many neurological diseases, such as Parkinson's disease, stroke, etc., and may be involved in processes such as inflammation and oxidative stress.