Recombinant Human A2M Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: A2M-499H
• Product Overview: A2M MS Standard C13 and N15-labeled recombinant protein (NP_000005) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 163.3 kDa
• AA Sequence: MGKNKLLHPSLVLLLLVLLPTDASV SGKPQYMVLVPSLLHTETTEKGCVL LSYLNETVTVSASLESVRGNRSLFT DLEAENDVLHCVAFAVPKSSSNEEV MFLTVQVKGPTQEFKKRTTVMVKNE DSLVFVQTDKSIYKPGQTVKFRVVS MDENFHPLNELIPLVYIQDPKGNRI AQWQSFQLEGGLKQFSFPLSSEPFQ GSYKVVVQKKSGGRTEHPFTVEEFV LPKFEVQVTVPKIITILEEEMNVSV CGLYTYGKPVPGHVTVSICRKYSDA SDCHGEDSQAFCEKFSGQLNSHGCF YQQVKTKVFQLKRKEYEMKLHTEAQ IQEEGTVVELTGRQSSEITRTITKL SFVKVDSHFRQGIPFFGQVRLVDGK GVPIPNKVIFIRGNEANYYSNATTD EHGLVQFSINTTNVMGTSLTVRVNY KDRSPCYGYQWVSEEHEEAHHTAYL VFSPSKSFVHLEPMSHELPCGHTQT VQAHYILNGGTLLGLKKLSFYYLIM AKGGIVRTGTHGLLVKQEDMKGHFS ISIPVKSDIAPVARLLIYAVLPTGD VIGDSAKYDVENCLANKVDLSFSPS QSLPASHAHLRVTAAPQSVCALRAV DQSVLLMKPDAELSASSVYNLLPEK DLTGFPGPLNDQDDEDCINRHNVYI NGITYTPVSSTNEKDMYSFLEDMGL KAFTNSKIRKPKMCPQLQQYEMHGP EGLRVGFYESDVMGRGHARLVHVEE PHTETVRKYFPETWIWDLVVVNSAG VAEVGVTVPDTITEWKAGAFCLSED AGLGISSTASLRAFQPFFVELTMPY SVIRGEAFTLKATVLNYLPKCIRVS VQLEASPAFLAVPVEKEQAPHCICA NGRQTVSWAVTPKSLGNVNFTVSAE ALESQELCGTEVPSVPEHGRKDTVI KPLLVEPEGLEKETTFNSLLCPSGG EVSEELSLKLPPNVVEESARASVSV LGDILGSAMQNTQNLLQMPYGCGEQ NMVLFAPNIYVLDYLNETQQLTPEV KSKAIGYLNTGYQRQLNYKHYDGSY STFGERYGRNQGNTWLTAFVLKTFA QARAYIFIDEAHITQALIWLSQRQK DNGCFRSSGSLLNNAIKGGVEDEVT LSAYITIALLEIPLTVTHPVVRNAL FCLESAWKTAQEGDHGSHVYTKALL AYAFALAGNQDKRKEVLKSLNEEAV KKDNSVHWERPQKPKAPVGHFYEPQ APSAEVEMTSYVLLAYLTAQPAPTS EDLTSATNIVKWITKQQNAQGGFSS TQDTVVALHALSKYGAATFTRTGKA AQVTIQSSGTFSSKFQVDNNNRLLL QQVSLPELPGEYSMKVTGEGCVYLQ TSLKYNILPEKEEFPFALGVQTLPQ TCDEPKAHTSFQISLSVSYTGSRSA SNMAIVDVKMVSGFIPLKPTVKMLE RSNHVSRTEVSSNHVLIYLDKVSNQ TLSLFFTVLQDVPVRDLKPAIVKVY DYYETDEFAIAEYNAPCSKDLGNAT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: A2M alpha-2-macroglobulin [ Homo sapiens (human) ]
• Official Symbol: A2M
• Synonyms: A2M; alpha-2-macroglobulin; CPAMD5; FWP007; S863 7; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; A2MD; S863-7; DKFZp779B086
• Gene ID: 2
• mRNA Refseq: NM_000014
• Protein Refseq: NP_000005
• MIM: 103950
• UniProt ID: P01023

Reviews

04/19/2022

It can be seen that the production process of A2M pays great attention to purity and biological activity, which makes its products have extremely high application value.

05/23/2020

Short half-life and high clearance of A2M make them more suitable for use in emergency medical situations and provide more treatment options for patients.

03/09/2020

The production process of A2M achieves efficient use of energy, reduces energy consumption, reduces carbon emissions, and is very environmentally friendly.

Q&As

What role does A2M protein play in the immune system?

It play an important role in the immune system, binding and neutralizing viruses, bacteria, reducing inflammation, and participating in immune regulation.

What is the relationship between A2M protein and lung disease?

Studies of the A2M protein in lung disease have shown that it may work by inhibiting inflammation and reducing lung tissue damage.

In which organs or tissues are A2M proteins expressed high?

The expression of A2M protein in organs or tissues such as liver, kidney, and lymphoid tissue is high.

How does A2M protein remove harmful proteins from the body?

Proteins eventually clear harmful proteins by capturing and binding to harmful proteins and then entering the cell through internalization.

Can A2M protein be used as a potential therapeutic target for cardiovascular disease?

A2M proteins show potential therapeutic targets in cardiovascular diseases and may have effects on cardiovascular diseases by modulating inflammatory responses and blood clotting.

In which neurological diseases are A2M proteins studied?

A2M proteins are studied in many neurological diseases, such as Parkinson's disease, stroke, etc., and may be involved in processes such as inflammation and oxidative stress.