Recombinant Human A2M Protein (Ser24-Ala1474), N-His tagged, Biotinylated

• Cat.No.: A2M-10H
• Product Overview: Biotin-conjugated recombinant human alpha-2-macroglobulin/A2M protein, derived from the transfected human HEK293 cells. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ser24-Ala1474
• Description: The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified.
• Form: Lyophilized powder
• Molecular Mass: Recombinant human A2M protein has a calculated molecular mass of 160 kDa. For unknown reason, the purified A2M protein can not enter SDS-PAGE gel. The identity of purified A2M protein was confirmed by Sandwich ELISA using specific antibodies.
• Purity: > 95%
• Storage: Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store desiccated below -20 centigrade in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 centigrade. For long term storage, it is recommended to make appropriate aliquots and store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.4)
• Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5-1.0 mg/mL with sterile deionized water.
• Shipping: Ice packs
• Conjugation: Biotin

Gene Information

• Gene Name: A2M alpha-2-macroglobulin [ Homo sapiens (human) ]
• Official Symbol: A2M
• Synonyms: A2M; alpha-2-macroglobulin; A2MD; CPAMD5; FWP007; S863-7; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; alpha-2-M
• Gene ID: 2
• mRNA Refseq: NM_000014
• Protein Refseq: NP_000005
• MIM: 103950
• UniProt ID: P01023