Recombinant Human A2M Protein (Ser24-Ala1474), N-His tagged, Biotinylated

• Cat.No.: A2M-10H
• Product Overview: Biotin-conjugated recombinant human alpha-2-macroglobulin/A2M protein, derived from the transfected human HEK293 cells. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.

Specification

• Description: The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized powder
• Molecular Mass: Recombinant human A2M protein has a calculated molecular mass of 160 kDa. For unknown reason, the purified A2M protein can not enter SDS-PAGE gel. The identity of purified A2M protein was confirmed by Sandwich ELISA using specific antibodies.
• Protein length: Ser24-Ala1474
• Purity: > 95%
• Storage: Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store desiccated below -20 centigrade in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 centigrade. For long term storage, it is recommended to make appropriate aliquots and store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.4)
• Reconstitution: Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5-1.0 mg/mL with sterile deionized water.
• Shipping: Ice packs

Gene Information

• Gene Name: A2M alpha-2-macroglobulin [ Homo sapiens (human) ]
• Official Symbol: A2M
• Synonyms: A2M; alpha-2-macroglobulin; A2MD; CPAMD5; FWP007; S863-7; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; alpha-2-M
• Gene ID: 2
• mRNA Refseq: NM_000014
• Protein Refseq: NP_000005
• MIM: 103950
• UniProt ID: P01023

Reviews

04/19/2022

It can be seen that the production process of A2M pays great attention to purity and biological activity, which makes its products have extremely high application value.

05/23/2020

Short half-life and high clearance of A2M make them more suitable for use in emergency medical situations and provide more treatment options for patients.

03/09/2020

The production process of A2M achieves efficient use of energy, reduces energy consumption, reduces carbon emissions, and is very environmentally friendly.

Q&As

What role does A2M protein play in the immune system?

It play an important role in the immune system, binding and neutralizing viruses, bacteria, reducing inflammation, and participating in immune regulation.

What is the relationship between A2M protein and lung disease?

Studies of the A2M protein in lung disease have shown that it may work by inhibiting inflammation and reducing lung tissue damage.

In which organs or tissues are A2M proteins expressed high?

The expression of A2M protein in organs or tissues such as liver, kidney, and lymphoid tissue is high.

How does A2M protein remove harmful proteins from the body?

Proteins eventually clear harmful proteins by capturing and binding to harmful proteins and then entering the cell through internalization.

Can A2M protein be used as a potential therapeutic target for cardiovascular disease?

A2M proteins show potential therapeutic targets in cardiovascular diseases and may have effects on cardiovascular diseases by modulating inflammatory responses and blood clotting.

In which neurological diseases are A2M proteins studied?

A2M proteins are studied in many neurological diseases, such as Parkinson's disease, stroke, etc., and may be involved in processes such as inflammation and oxidative stress.