Recombinant Human DPH5 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human DPH5 Protein, Myc/DDK-tagged, C13 and N15-labeled

DPH5 Related Products

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Cat.No. : DPH5-6497H
Product Overview : DPH5 MS Standard C13 and N15-labeled recombinant protein (NP_057042) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 31.5 kDa
AA Sequence : MLYLIGLGLG DAKDITVKGL EVVRRCSRVY LEAYTSVLTV GKEALEEFYG RKLVVADREE VEQEADNILK DADISDVAFL VVGDPFGATT HSDLVLRATK LGIPYRVIHN ASIMNAVGCC GLQLYKFGET VSIVFWTDTW RPESFFDKVK KNRQNGMHTL CLLDIKVKEQ SLENLIKGRK IYEPPRYMSV NQAAQQLLEI VQNQRIRGEE PAVTEETLCV GLARVGADDQ KIAAGTLRQM CTVDLGEPLH SLIITGGSIH PMEMEMLSLF SIPENSSESQ SINGLTRTRP LEQKLISEED LAANDILDYK DDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : DPH5 diphthamide biosynthesis 5 [ Homo sapiens (human) ]
Official Symbol : DPH5
Synonyms : DPH5; DPH5 homolog (S. cerevisiae); diphthine synthase; CGI 30; protein x 0011; diphthamide biosynthesis methyltransferase; AD-018; CGI-30; NPD015; HSPC143; MGC61450;
Gene ID : 51611
mRNA Refseq : NM_015958
Protein Refseq : NP_057042
MIM : 611075
UniProt ID : Q9H2P9

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