Recombinant Human DPH5 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DPH5-6497H
• Product Overview: DPH5 MS Standard C13 and N15-labeled recombinant protein (NP_057042) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 31.5 kDa
• AA Sequence: MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DPH5 diphthamide biosynthesis 5 [ Homo sapiens (human) ]
• Official Symbol: DPH5
• Synonyms: DPH5; DPH5 homolog (S. cerevisiae); diphthine synthase; CGI 30; protein x 0011; diphthamide biosynthesis methyltransferase; AD-018; CGI-30; NPD015; HSPC143; MGC61450
• Gene ID: 51611
• mRNA Refseq: NM_015958
• Protein Refseq: NP_057042
• MIM: 611075
• UniProt ID: Q9H2P9