| Cat.No. : |
EGFR-339H |
| Product Overview : |
Recombinant Human EGFR Protein, Fc-tagged is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # NP_005219). Predicted N-terminus: Leu 25. This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 95 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Fc |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Human EGF, Tag Free at 10μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein, Fc-tagged with a linear range of 0.2-3.2 μg/mL (QC tested).
Recombinant Human EGFR Protein, Fc-tagged at 1 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.1-2 ng/mL (Routinely tested). |
| Molecular Mass : |
95 kDa |
| Protein length : |
Leu 25 - Ser 645 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Notes : |
Please avoid repeated freeze-thaw cycles. |
| Stability : |
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
| Figure : |
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