Recombinant Human FGF23 293 Cell Lysate

Cat.No. : FGF23-6241HCL
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Description : Antigen standard for fibroblast growth factor 23 (FGF23) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : FGF23 fibroblast growth factor 23 [ Homo sapiens ]
Official Symbol : FGF23
Synonyms : FGF23; fibroblast growth factor 23;
Gene ID : 53406

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What role does FGF23 play in chronic kidney disease? 06/12/2022

FGF23 levels are elevated in chronic kidney disease, contributing to mineral imbalance.

How is FGF23 implicated in cardiovascular health? 04/04/2022

High FGF23 levels are associated with increased cardiovascular risk.

How does FGF23 regulate phosphate and calcium homeostasis? 03/24/2022

It decreases renal phosphate reabsorption and 1,25-dihydroxyvitamin D production.

Are there known genetic mutations that affect FGF23 production or activity? 01/27/2022

Mutations in FGF23 or related proteins can lead to dysregulated phosphate metabolism.

What therapeutic strategies are being explored to modulate FGF23 levels? 05/16/2020

Strategies include phosphate binders and FGF23 antibodies to manage elevated FGF23 levels.

In which tissues is FGF23 predominantly produced and secreted? 04/28/2020

FGF23 is primarily produced in bone.

What is the primary function of FGF23 in the body? 10/29/2019

FGF23 regulates phosphate and vitamin D metabolism.

Customer Reviews (3)

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Reviews
07/23/2022

    Excellent results, high purity.

    07/17/2021

      Reliable and effective product.

      11/26/2020

        Quick delivery, product works.

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