Recombinant Human GFAP protein, His & S-tagged

• Cat.No.: GFAP-885H
• Product Overview: Recombinant Human GFAP aa. (Glu254~Glu374 (Accession# P14136)) fused with N-terminal His & S tag was produced in E. coli cells.

Specification

• Description: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: E. coli
• Species: Human
• Tag: His & S
• Form: Freeze-dried powder
• Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Glu254~Glu374 (Accession# P14136)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 5.1.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: GFAP glial fibrillary acidic protein [ Homo sapiens (human) ]
• Official Symbol: GFAP
• Synonyms: GFAP; glial fibrillary acidic protein; ALXDRD; glial fibrillary acidic protein
• Gene ID: 2670
• mRNA Refseq: NM_001131019.2
• Protein Refseq: NP_001124491.1
• UniProt ID: P14136