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Recombinant Human GPC3 Protein (S359F), His-tagged, low endotoxin, FITC conjugated
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June 03, 2023 | Add to Quote |
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Cat.No. : | GPC3-2246HF Optional Service: Optional requirements on this protein |
Product Overview : | FITC conjugated recombinant human GPC3 (S359F, Gln25-His559)(low endotoxin) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293). |
Description : | Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3/GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. |
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized |
Molecular Mass : | The protein has a calculated MW of 61.7 kDa. The protein migrates as 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : | Gln25-His559 |
N-terminal Sequence Analysis : | Gln 25 |
Endotoxin : | < 0.1 EU/ μg by the LAL method. |
Purity : | > 90 % as determined by SDS-PAGE |
Characteristic : | Disulfide-linked homodimer Labeled with FITC via amines Excitation source: 488 nm spectral line, argon-ion laser Excitation Wavelength: 488 nm Emission Wavelength: 535 nm |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | GPC3 |
Official Symbol : | GPC3 |
Synonyms : | GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2 |
Gene ID : | 2719 |
mRNA Refseq : | NM_001164617 |
Protein Refseq : | NP_001158089 |
MIM : | 300037 |
UniProt ID : | P51654 |
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