IL12A-062H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35, aa23-219) and 40 kDa (p40, aa23-328) subunits (503 total amino acid residues) without tag was expressed in E. coli.
Description :
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
Source :
E. coli
Species :
Human
Bio-activity :
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL.
Molecular Mass :
Recombinant Human IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35, aa23-219) and 40 kDa (p40, aa23-328) subunits (503 total amino acid residues).
Endotoxin :
< 1.0 EU/μg of the protein as determined by the LAL method
Purity :
> 95 % as determined by SDS-PAGE
Storage :
At -80 centigrade. Avoid repeated freeze-thaw cycles.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.