Recombinant Human IL13 protein
| Cat.No. : | IL13-22H |
| Product Overview : | Recombinant Human IL13 protein was expressed in Escherichia coli. |
- Specification
- Gene Information
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| Description : | Human Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 5 and secreted by many cell types, especially T helper type 2 (Th2) cells. The high solution from of IL-13 reported to be a monomer with two internal disulfide bonds that contribute to a bundled four α-helix configuration. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta, TNF-alpha, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Human, mouse and rat IL-3 share low homology, but have cross species activity. |
| Source : | E.coli |
| Species : | Human |
| Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4 with 5 % trehalose. |
| Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg. |
| Molecular Mass : | Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 112 amino acids. |
| Protein length : | 112 |
| AA Sequence : | GPVPPSTALRELIEELVNITQNQKA PLCNGSMVWSINLTAGMYCAALESL INVSGCSAIEKTQRMLSGFCPHKVS AGQFSSLHVRDTKIEVAQFVKDLLL HLKKLFREGRFN |
| Endotoxin : | Less than 1 EU/µg of rHuIL-13 as determined by LAL method. |
| Purity : | >97% by SDS-PAGE and HPLC analysis. |
| Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 20 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
| Gene Name : | IL13 |
| Official Symbol : | IL13 |
| Synonyms : | IL13; interleukin 13; interleukin-13; allergic rhinitis; ALRH; BHR1; Bronchial hyperresponsiveness 1 (bronchial asthma); IL 13; MGC116786; MGC116788; MGC116789; P600; Bronchial hyperresponsiveness-1 (bronchial asthma); IL-13; |
| Gene ID : | 3596 |
| mRNA Refseq : | NM_002188 |
| Protein Refseq : | NP_002179 |
| MIM : | 147683 |
| UniProt ID : | P35225 |
Products Types
| ◆ Recombinant Protein | ||
| Il13-087M | Active Recombinant Mouse Il13 Protein | +Inquiry |
| IL13-1660R | Recombinant Rhesus monkey IL13 Protein, His-tagged | +Inquiry |
| IL13-179H | Active Recombinant Human IL13 Protein (Gly35-Asn146), C-His tagged, Animal-free, Carrier-free | +Inquiry |
| IL13-4350R | Recombinant Rabbit IL13 Protein | +Inquiry |
| Il13-256I | Active Recombinant Mouse Il13 Protein, His-tagged | +Inquiry |
| ◆ Lysates | ||
| IL13-1336MCL | Recombinant Mouse IL13 cell lysate | +Inquiry |
| IL13-2922HCL | Recombinant Human IL13 cell lysate | +Inquiry |
| IL13-1894CCL | Recombinant Cynomolgus IL13 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
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Q&As (10)
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How does flow cytometry help quantify IL13 receptor expression on different cell populations?
12/29/2022
Flow cytometry quantifies IL13 receptor expression on cells.
What are the benefits of using transgenic animal models to study IL13's in vivo functions?
07/05/2022
Transgenic models illuminate IL13's in vivo functions.
Could you explain the role of ELISA in quantifying IL13 secretion levels in cell cultures?
02/23/2022
ELISA quantifies IL13 secretion in cell cultures accurately.
How can siRNA knockdown experiments reveal IL13's effects on specific cellular processes?
06/13/2021
siRNA knockdown uncovers IL13's specific cellular effects.
How does co-immunoprecipitation identify interacting partners in IL13-mediated signaling complexes?
07/28/2019
Co-immunoprecipitation identifies IL13 signaling partners.
What techniques can differentiate IL13-mediated signaling pathways from IL4 pathways?
07/23/2019
Differential techniques distinguish IL13 and IL4 signaling pathways.
Could you elaborate on the use of reporter gene assays to assess IL13-driven transcriptional activity?
12/16/2018
Reporter gene assays gauge IL13-driven transcription.
Can you describe the applications of microarray analysis in profiling IL13-induced gene expression changes?
11/14/2018
Microarray analysis profiles IL13-induced gene expression.
What experimental methods determine the impact of IL13 on tissue remodeling processes?
08/04/2018
Experimental methods reveal IL13's impact on tissue remodeling.
How does site-directed mutagenesis help identify critical residues in IL13 receptor binding?
07/04/2018
Site-directed mutagenesis identifies key residues in IL13 receptor binding.
Customer Reviews (3)
Write a reviewReviews
11/29/2021
Effective in Th2 cell differentiation. -
04/22/2020
Good for cytokine assays. -
01/26/2019
High yield in expression. -
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