Recombinant Human IL13 protein
|Product Overview :||Recombinant Human IL13 protein was expressed in Escherichia coli.|
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|Description :||Human Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 5 and secreted by many cell types, especially T helper type 2 (Th2) cells. The high solution from of IL-13 reported to be a monomer with two internal disulfide bonds that contribute to a bundled four α-helix configuration. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta, TNF-alpha, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Human, mouse and rat IL-3 share low homology, but have cross species activity.|
|Form :||Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4 with 5 % trehalose.|
|Bio-activity :||Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.|
|Molecular Mass :||Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 112 amino acids.|
|Protein length :||112|
|AA Sequence :||GPVPPSTALRELIEELVNITQNQKA PLCNGSMVWSINLTAGMYCAALESL INVSGCSAIEKTQRMLSGFCPHKVS AGQFSSLHVRDTKIEVAQFVKDLLL HLKKLFREGRFN|
|Endotoxin :||Less than 1 EU/µg of rHuIL-13 as determined by LAL method.|
|Purity :||>97% by SDS-PAGE and HPLC analysis.|
|Storage :||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.|
|Reconstitution :||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 20 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.|
|Gene Name :||IL13|
|Official Symbol :||IL13|
|Synonyms :||IL13; interleukin 13; interleukin-13; allergic rhinitis; ALRH; BHR1; Bronchial hyperresponsiveness 1 (bronchial asthma); IL 13; MGC116786; MGC116788; MGC116789; P600; Bronchial hyperresponsiveness-1 (bronchial asthma); IL-13;|
|Gene ID :||3596|
|mRNA Refseq :||NM_002188|
|Protein Refseq :||NP_002179|
|UniProt ID :||P35225|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Flow cytometry quantifies IL13 receptor expression on cells.
Transgenic models illuminate IL13's in vivo functions.
ELISA quantifies IL13 secretion in cell cultures accurately.
siRNA knockdown uncovers IL13's specific cellular effects.
Co-immunoprecipitation identifies IL13 signaling partners.
Differential techniques distinguish IL13 and IL4 signaling pathways.
Reporter gene assays gauge IL13-driven transcription.
Microarray analysis profiles IL13-induced gene expression.
Experimental methods reveal IL13's impact on tissue remodeling.
Site-directed mutagenesis identifies key residues in IL13 receptor binding.
Customer Reviews (3)Write a review
Effective in Th2 cell differentiation. -
Good for cytokine assays. -
High yield in expression. -
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