Recombinant Human MAPK8 protein(Met1-Arg427), GST-tagged

• Cat.No.: MAPK8-781H
• Product Overview: Recombinant Human MAPK8 isoform JNK1 alpha 2 (NP_620637.1) (Met 1-Arg 427) was expressed in Insect Cells with the GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-GST
• Protein length: Met1-Arg427
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 25% glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human MAPK8/GST chimera consists of 652 amino acids and predicts a molecular mass of 75 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MAPK8 mitogen-activated protein kinase 8 [ Homo sapiens ]
• Official Symbol: MAPK8
• Synonyms: MAPK8; mitogen-activated protein kinase 8; PRKM8; JNK; JNK1; JUN N terminal kinase; SAPK1; MAP kinase 8; JUN N-terminal kinase; c-Jun N-terminal kinase 1; stress-activated protein kinase 1; stress-activated protein kinase 1c; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8 isoform JNK1 alpha1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• Gene ID: 5599
• mRNA Refseq: NM_002750
• Protein Refseq: NP_002741
• MIM: 601158
• UniProt ID: P45983