Recombinant Human RYR1 Protein (1-534 aa), His-tagged

• Cat.No.: RYR1-789H
• Product Overview: Recombinant Human RYR1 Protein (1-534 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Transport. Protein Description: Partial.

Specification

• Description: Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca2+ leaking into the cytoplasm. Can also mediate the release of Ca2+ from intracellular stores in neurons, and may thereby promote prolonged Ca2+ signaling in the brain. Required for normal bryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during bryogenesis.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 63.3 kDa
• Protein length: 1-534 aa
• AA Sequence: MGDAEGEDEVQFLRTDDEVVLQCSA TVLKEQLKLCLAAEGFGNRLCFLEP TSNAQNVPPDLAICCFVLEQSLSVR ALQEMLANTVEAGVESSQGGGHRTL LYGHAILLRHAHSRMYLSCLTTSRS MTDKLAFDVGLQEDATGEACWWTMH PASKQRSEGEKVRVGDDIILVSVSS ERYLHLSTASGELQVDASFMQTLWN MNPICSRCEEGFVTGGHVLRLFHGH MDECLTISPADSDDQRRLVYYEGGA VCTHARSLWRLEPLRISWSGSHLRW GQPLRVRHVTTGQYLALTEDQGLVV VDASKAHTKATSFCFRISKEKLDVA PKRDVEGMGPPEIKYGESLCFVQHV ASGLWLTYAAPDPKALRLGVLKKKA MLHQEGHMDDALSLTRCQQEESQAA RMIHSTNGLYNQFIKSLDSFSGKPR GSGPPAGTALPIEGVILSLQDLIIY FEPPSEDLQHEEKQSKLRSLRNRQS LFQEEGMLSMVLNCIDRLNVYTTAA HFAEFAGEEAAESWKEIVNLLYELL ASLIRGNRS
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: RYR1 ryanodine receptor 1 [ Homo sapiens (human) ]
• Official Symbol: RYR1
• Synonyms: CCO; MHS; RYR; MHS1; RYDR; SKRR; RYR-1; PPP1R137
• Gene ID: 6261
• UniProt ID: P21817