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Recombinant Human SOCS1 293 Cell Lysate

Cat.No. : SOCS1-1582HCL
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Description : Antigen standard for suppressor of cytokine signaling 1 (SOCS1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : SOCS1 suppressor of cytokine signaling 1 [ Homo sapiens ]
Official Symbol : SOCS1
Synonyms : SOCS1; suppressor of cytokine signaling 1; Cish1; JAB; SOCS 1; SSI 1; TIP3; TIP-3; JAK binding protein; JAK-binding protein; Tec-interacting protein 3; STAT induced SH3 protein 1; STAT-induced STAT inhibitor 1; cytokine-inducible SH2 protein 1; CIS1; SSI1; CISH1; SSI-1; SOCS-1;
Gene ID : 8651
mRNA Refseq : NM_003745
Protein Refseq : NP_003736
MIM : 603597
UniProt ID : O15524
Chromosome Location : 16p13.13
Pathway : Adaptive Immune System, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem;
Function : insulin-like growth factor receptor binding; kinase inhibitor activity; protein binding; protein kinase binding; protein kinase inhibitor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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