SRC-004H
Optional Service: Optional requirements on this protein
Product Overview :
Active recombinant full length human SRC Protein (1-536) with a N-terminal GST tag was expressed in insect cells.
Description :
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Source :
Insect cell
Species :
Human
Tag :
GST
Bio-activity :
Tris buffered solution containing MgCl2, 10μM ATP, 10 μg Poly-Glu, Tyr 4:1 substrate and 30 ng of Src. Incubate at 30 centigrade for 45 min, and measure ATP via luminescent assay. Luminescence is inversely correlated to kinase activity.
Molecular Mass :
86 kDa
Protein length :
1-536
Purity :
≥60% as determined by SDS-PAGE
Applications :
Enzyme assay
Stability :
≥ 6 months
Storage :
At -80 centigrade.
Storage Buffer :
25 mM Tris-HCl, pH 8.0, with 69 mM sodium chloride, 1.35 mM potassium chloride, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.