Active Recombinant Human SRC Protein, GST-tagged

• Cat.No.: SRC-004H
• Product Overview: Active recombinant full length human SRC Protein (1-536) with a N-terminal GST tag was expressed in insect cells.

Specification

• Description: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
• Source: Insect cell
• Species: Human
• Tag: GST
• Bio-activity: Tris buffered solution containing MgCl2, 10μM ATP, 10 μg Poly-Glu, Tyr 4:1 substrate and 30 ng of Src. Incubate at 30 centigrade for 45 min, and measure ATP via luminescent assay. Luminescence is inversely correlated to kinase activity.
• Molecular Mass: 86 kDa
• Protein length: 1-536
• Purity: ≥60% as determined by SDS-PAGE
• Applications: Enzyme assay
• Stability: ≥ 6 months
• Storage: At -80 centigrade.
• Storage Buffer: 25 mM Tris-HCl, pH 8.0, with 69 mM sodium chloride, 1.35 mM potassium chloride, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT

Gene Information

• Gene Name: SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: SRC
• Synonyms: SRC; SRC proto-oncogene, non-receptor tyrosine kinase; ASV; SRC1; THC6; c-SRC; p60-Src; proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; EC 2.7.10.2
• Gene ID: 6714
• mRNA Refseq: NM_005417
• Protein Refseq: NP_005408
• MIM: 190090
• UniProt ID: P12931