Recombinant Human SRC Protein, GST-tagged
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Cat.No. : | SRC-004H |
Product Overview : | Active recombinant full length human SRC Protein (1-536) with a N-terminal GST tag was expressed in insect cells. |
Description : | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
Source : | Insect cell |
Species : | Human |
Tag : | GST |
Bio-activity : | Tris buffered solution containing MgCl2, 10μM ATP, 10 μg Poly-Glu, Tyr 4:1 substrate and 30 ng of Src. Incubate at 30 centigrade for 45 min, and measure ATP via luminescent assay. Luminescence is inversely correlated to kinase activity. |
Molecular Mass : | 86 kDa |
Protein length : | 1-536 |
Purity : | ≥60% as determined by SDS-PAGE |
Applications : | Enzyme assay |
Stability : | ≥ 6 months |
Storage : | At -80 centigrade. |
Storage Buffer : | 25 mM Tris-HCl, pH 8.0, with 69 mM sodium chloride, 1.35 mM potassium chloride, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT |
SDS-PAGE analysis of SRC : |
![]() Lane 1: SRC (4 μg) |
SRC (10 μM ATP) : |
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Specific Activity : |
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Gene Name : | SRC SRC proto-oncogene, non-receptor tyrosine kinase [ Homo sapiens (human) ] |
Official Symbol : | SRC |
Synonyms : | SRC; SRC proto-oncogene, non-receptor tyrosine kinase; ASV; SRC1; THC6; c-SRC; p60-Src; proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; EC 2.7.10.2 |
Gene ID : | 6714 |
mRNA Refseq : | NM_005417 |
Protein Refseq : | NP_005408 |
MIM : | 190090 |
UniProt ID : | P12931 |
◆ Recombinant Protein
SRC-2747H | Recombinant Human SRC Protein, MYC/DDK-tagged | +Inquiry |
Src-6122M | Recombinant Mouse Src Protein, Myc/DDK-tagged | +Inquiry |
SRC-533H | Recombinant Human SRC protein, His-tagged | +Inquiry |
SRC-1128Z | Recombinant Zebrafish SRC | +Inquiry |
Src-546R | Recombinant RSV Src, GST-tagged | +Inquiry |
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◆ Native Protein
SRC-29697TH | Native Human SRC | +Inquiry |
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◆ Lysates
SRC-487HCL | Recombinant Human SRC cell lysate | +Inquiry |
SRC-585MCL | Recombinant Mouse SRC cell lysate | +Inquiry |
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