Active Recombinant Mouse Cd4 protein(Met1-Thr394), His-tagged

• Cat.No.: Cd4-2276M
• Product Overview: Recombinant Mouse CD4 (NP_038516.1) (Met 1-Thr 394) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Thr394
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well are added to mCD4-His coated plates (0.2μg/mL and 100μL/well), approximately 30% will adhere specifically after 30 minutes at 37°C.
• Molecular Mass: The recombinant mouse CD4 consists of 379 amino acids and has a predicted molecular mass of 42.8 kDa. As a result of glycosylation, the apparent molecular mass of rmCD4 is approximately 50-55 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd4 CD4 antigen [ Mus musculus ]
• Official Symbol: Cd4
• Synonyms: CD4; CD4 antigen; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; T-cell differentiation antigen L3T4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); L3T4; Ly-4
• Gene ID: 12504
• mRNA Refseq: NM_013488
• Protein Refseq: NP_038516