| Cat.No. : |
Il27-3518M
Optional Service: Optional requirements on this protein
|
| Product Overview : |
Purified recombinant protein of Mouse interleukin 27 (Il27) without tag was expressed in HEK293. |
| Description : |
Broad expression in genital fat pad adult (RPKM 5.8), spleen adult (RPKM 2.5) and 18 other tissues |
| Source : |
HEK293 |
| Species : |
Mouse |
| Bio-activity : |
The ED50 is 5 - 10 ng/mL, corresponding to a specific activity of 1.0 - 2.0 × 10^5 units/mg, determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies. |
| Molecular Mass : |
49 kDa |
| Endotoxin : |
Less than 0.01 ng/μg protein as determined by the LAL method |
| Purity : |
> 95%, as determined by Coomassie stained SDS-PAGE. |
| Stability : |
Unopened vial can be stored between 2 and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
Protein formulated and vialed at >50 μg/mL in specified buffer |
| Storage Buffer : |
20 mM NaH2PO4, pH 6, 0.60 M NaCl |
| SDS-PAGE : |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
