| Species : |
Mouse |
| Source : |
HEK293 |
| Protein Length : |
23-140 a.a. |
| Description : |
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. |
| Bio-activity : |
The ED50 is 2 - 4 ng/ml, corresponding to a specific activity of 2.5 - 5 x 10^5 units/mg, determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose dependent manner. |
| Molecular Mass : |
13.3 kDa |
| Endotoxin : |
Less than 0.01 ng per µg protein as determined by the LAL method |
| Purity : |
>95%, as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
Protein formulated and vialed at >50 ug/mL in specified buffer |
| Storage Buffer : |
1 x PBS |
