Recombinant Rat Cntf Protein, His-tagged
Cat.No. : | Cntf-374R |
Product Overview : | Recombinant rat CNTF, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
- Specification
- Gene Information
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Description : | CNTF also known as ciliary neurotrphic factor is a survival factor for various neuronal cell types. CNTF is highly conserved across species and exhibits cross-species bioactivity. CNTF is a 25 kDa polypeptide that was originally isolated as a target-derived survival factor of parasympathetic ciliary ganglion neurons. Binding of CNTF to CNTF receptor triggers heterodimerization of gp130 and LIFR, forming an active trimeric receptor complex and activates the downstream signaling pathway. |
Source : | E.coli |
Species : | Rat |
Tag : | His |
Form : | Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol. |
Molecular Mass : | 25.2 kDa (223aa) confirmed by MALDI-TOF |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSMAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKN INLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVS AFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGIS ALESHYGAKD KQM |
Endotoxin : | < 1.0 EU per 1 microgram of protein (determined by LAL method) |
Purity : | >95% by SDS-PAGE |
Storage : | Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5 mg/ml (determined by Bradford assay) |
Gene Name : | Cntf ciliary neurotrophic factor [ Rattus norvegicus ] |
Official Symbol : | Cntf |
Synonyms : | CNTF; ciliary neurotrophic factor; ciliary neurotropic factor; |
Gene ID : | 25707 |
mRNA Refseq : | NM_013166 |
Protein Refseq : | NP_037298 |
MIM : | |
UniProt ID : | |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; |
Function : | ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; growth factor activity; interleukin-6 receptor binding; interleukin-6 receptor binding; |
Products Types
◆ Native Protein | ||
CNTF-26839TH | Native Human CNTF | +Inquiry |
◆ Lysates | ||
CNTF-376HCL | Recombinant Human CNTF cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionCNTF plays a crucial role in regulating glial cell function. It can modulate astrocyte and oligodendrocyte differentiation, promote myelination, and regulate the release of neurotrophic factors from glial cells. CNTF also influences glial cell-mediated immune responses and their interaction with neurons in various pathological conditions.
CNTF activates multiple signaling pathways, including JAK/STAT, PI3K/AKT, and MAPK/ERK, in both neurons and glial cells. These pathways play crucial roles in regulating gene expression, cell survival, differentiation, and synaptic plasticity.
CNTF plays a significant role in modulating neuroinflammatory responses. It can regulate the activation and function of microglia and astrocytes, leading to the production of pro-inflammatory or anti-inflammatory factors. CNTF also influences the recruitment and activation of immune cells in the central nervous system.
CNTF has been shown to modulate synaptic plasticity, which underlies learning and memory processes. It promotes the formation and stabilization of synapses, regulates neurotransmitter release, and influences synaptic strength and plasticity through its interaction with specific receptors and downstream signaling molecules.
Developing CNTF-based therapies faces challenges such as the need for targeted delivery to specific brain regions, potential side effects due to systemic administration, and the need to optimize dosing and treatment regimens. Additionally, understanding the precise mechanisms of CNTF action and identifying suitable patient populations for treatment are important considerations.
CNTF exerts neuroprotective effects by promoting neuronal survival and regeneration through the activation of various intracellular signaling pathways. It enhances the expression of neurotrophic factors, regulates apoptosis-related genes, and stimulates axonal outgrowth and synaptic remodeling.
CNTF shows promise as a potential therapeutic approach for neurodegenerative diseases, such as amyotrophic lateral sclerosis and retinal degeneration. Its neuroprotective and neurotrophic effects make it a potential candidate for promoting neuronal survival, preventing neurodegeneration, and improving functional recovery in these conditions.
Customer Reviews (3)
Write a reviewIt is highly durable and meets the requirements for long-term experiments.
A trusted aide, this reagent is instrumental on the path of scientific inquiry.
Employing this protein reagent allows me to obtain precise structural information in protein interaction studies, offering critical insights for a deeper understanding of protein function.
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