"EFNB2" Related Products

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Recombinant Rat EFNB2 Protein, Fc-tagged

Cat.No.: EFNB2-1078R
Product Overview: Recombinant rat EFNB2 (NP_001100798.1) extracellular domain (Met 1-Ala 229) was fused with the Fc region of human IgG1 at the C-terminus.
Source: HEK293
Species: Rat
Tag: Fc
Predicted N Terminal: Ser 28
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EPHB4-His at 10 μg/mL (100 μl/well) can bind Rat EphrinB2-Fc, The EC50 of Rat EphrinB2-Fc is 10 ng/mL.
Molecular Mass: The recombinant rat EFNB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 443 amino acids and predicts a molecular mass of 49 kDa. The apparent molecular mass of the rat EFNB2/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Efnb2 ephrin B2 [ Rattus norvegicus ]
Official Symbol: EFNB2
Synonyms: EFNB2; ephrin B2;
Gene ID: 306636
mRNA Refseq: NM_001107328
Protein Refseq: NP_001100798
MIM:
UniProt ID:
Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem;
Function: ephrin receptor binding; ephrin receptor binding; receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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