Recombinant Rat Smad1 protein, His-tagged

• Cat.No.: Smad1-658R
• Product Overview: Recombinant Rat Smad1 aa. (His273~Ser468 (Accession # P97588)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: A transcriptional activator; activated by bone morphogenetic protein 4 (BMP4).
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions
• Protein length: His273~Ser468 (Accession # P97588)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Smad1 SMAD family member 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Smad1
• Synonyms: Smad1; SMAD family member 1; Madh1; mothers against decapentaplegic homolog 1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog1 (mothers against decapentaplegic, Drosophila); SMAD 1; SMAD, mothers against DPP homolog 1; mothers against DPP homolog 1
• Gene ID: 25671
• mRNA Refseq: NM_013130.2
• Protein Refseq: NP_037262.2
• UniProt ID: P97588

Reviews

06/08/2022

Whether I am investigating enzymatic activity, studying protein-protein interactions, or testing its role in signaling pathways, the SMAD1 protein consistently performs exceptionally well.

08/08/2021

This versatility allows me to explore different aspects of my research with confidence and accuracy.

05/02/2018

The SMAD1 protein's excellent functionality makes it highly suitable for various trial applications.

Q&As

What technologies are commonly used to study the expression and activity of SMAD1 in clinical research?

Techniques like Western blotting, immunohistochemistry, and qRT-PCR are commonly employed to study SMAD1 expression and activity in clinical research.

How might targeting SMAD1 have implications for anti-cancer therapies?

Modulating SMAD1 activity could be explored as a potential strategy for developing anti-cancer therapies, particularly in cancers where the TGF-β pathway is dysregulated.

Are there any known genetic mutations in the SMAD1 gene associated with human diseases?

Yes, mutations in the SMAD1 gene have been identified in certain individuals with congenital disorders and developmental abnormalities.

How does SMAD1 interact with other proteins in the TGF-β pathway?

SMAD1 forms complexes with other SMAD proteins, transducing signals from TGF-β receptors to the nucleus, where they regulate gene expression.

How can SMAD1 be targeted for therapeutic interventions in bone-related disorders?

Targeting SMAD1 or its downstream effectors in the TGF-β pathway may offer potential therapeutic strategies for bone-related disorders, such as osteoporosis.