vcam1
Share
  • Official Full Name
  • vascular cell adhesion molecule 1
  • Background
  • Human Vascular Cell Adhesion Molecule-1 is a 100 - 110 kDa, 715 amino acid type I transmembrane glycoprotein typically characterized by the presence of seven C2-type immunoglobulin domains. Its extracellular region is 674 aa in length, followed by a 22 aa transmembrane segment and a 19 aa cytoplasmic tail. In the extracellular region, there are multiple N-linked glycosylation sites, and each C2 domain is closed by a disulfide bridge. There is considerable interspecies VCAM-1 homology, with mouse and rat VCAM-1 showing approximately 75% aa identity to human VCAM-1. Notably, the short 19 aa cytoplasmic tail is absolutely conserved, mouse to human to rat. Cells expressing mouse VCAM-1 bind both mouse and human leukocytes, and this reflects their high degree of aa identity. A number of variants of VCAM-1 are known to occur, all of which are likely the result of alternate gene splicing. In particular, a human six Ig domain molecule is known, and in rabbits, an eight Ig domain form has been identified. There is also a three-C2 domain, 43 kDa GPI-linked form of VCAM-1. Although it binds known VCAM-1 ligands, its function is unclear. Cells known to express VCAM-1 include neurons, endothelial cells, smooth muscle cells, fibroblasts and macrophages.
    Soluble VCAM-1 has been identified in culture supernates, blood, and cerebrospinal fluid. In vitro, basal levels of VCAM-1 shedding by unstimulated NIH3T3 cells appear to partially require metalloproteinase activity, while PMA-induced shedding is dependent upon the proteolytic activity of TACE/ADAM17.
    Functionally, VCAM-1 binds to both 4 1 and 4 7 integrins. These integrins are expressed on a variety of cells, with VLA-4 found on all leukocytes with the exception of neutrophils. Because of this, VCAM-1/VCAM-1 ligand interactions are undoubtedly key events in the rate and timing of leukocyte extravasation. Other roles proposed for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia.
    The Human sVCAM-1 Immunoassay is a 2 hour solid-phase ELISA designed to measure human sVCAM-1 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sVCAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sVCAM-1 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that the Human sVCAM-1 immunoassay kit can be used to determine relative mass values for naturally occurring sVCAM-1.
  • Synonyms
  • VCAM1; vascular cell adhesion molecule 1; INCAM-100; Vascular cell adhesion protein 1 precursor; CD-10^6 antigen; CD10^6; DKFZp779G2333; MGC99561; CD10^6 antigen; OTTHUMP00000012650; OTTHUMP00000012651; L1CAM; V-CAM 1; CD106; Vcam-1
Cat.#:VCAM1-1793MATag:MIgG2a Fc
Source (Host):CHO CellsSpecies:Mouse
Product nameRecombinant Mouse VCAM1 protein, MIgG2a Fc-tagged, APC labeled
+Inquiry
Cat.#:VCAM1-1793MPTag:MIgG2a Fc
Source (Host):CHO CellsSpecies:Mouse
Product nameRecombinant Mouse VCAM1 protein, MIgG2a Fc-tagged, R-PE labeled
+Inquiry
Cat.#:VCAM1-1794MATag:HIgG1 Fc
Source (Host):CHO CellsSpecies:Human
Product nameRecombinant Human VCAM1 protein, HIgG1 Fc-tagged, APC labeled
+Inquiry
Cat.#:VCAM1-1794MPTag:HIgG1 Fc
Source (Host):CHO CellsSpecies:Human
Product nameRecombinant Human VCAM1 protein, HIgG1 Fc-tagged, R-PE labeled
+Inquiry
Cat.#:Vcam1-2313MATag:Fc|His
Source (Host):HEK293Species:Mouse
Product nameRecombinant Mouse Vcam1 protein, Fc-His-tagged, APC labeled
+Inquiry
Cat.#:Vcam1-2313MPTag:Fc|His
Source (Host):HEK293Species:Mouse
Product nameRecombinant Mouse Vcam1 protein, Fc-His-tagged, R-PE labeled
+Inquiry
Cat.#:VCAM1-71HATag:Fc
Source (Host):HEK293Species:Human
Product nameRecombinant Human VCAM1 protein, Fc-tagged, APC labeled
+Inquiry
Cat.#:VCAM1-71HPTag:Fc
Source (Host):HEK293Species:Human
Product nameRecombinant Human VCAM1 protein, Fc-tagged, R-PE labeled
+Inquiry
Cat.#:VCAM1-117HTag:Myc|DDK
Source (Host):HEK293Species:Human
Product nameRecombinant Human VCAM1 Protein, MYC/DDK-tagged
+Inquiry
Cat.#:VCAM1-170HTag:His
Source (Host):NS0Species:Human
Product nameRecombinant Human VCAM1 protein, His-tagged
+Inquiry
Cat.#:VCAM1-1794MTag:Fc
Source (Host):CHOSpecies:Human
Product nameRecombinant Human VCAM1, HIgG1 Fc-tagged
+Inquiry
Cat.#:VCAM1-179HTag:T7
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human VCAM1 protein, T7-tagged
+Inquiry
Cat.#:VCAM1-191HTag:N/A
Source (Host):HEK293Species:Human
Product nameRecombinant Human VCAM1 Protein
+Inquiry
Cat.#:VCAM1-2325HTag:His
Source (Host):HEK293Species:Human
Product nameRecombinant Human VCAM1, His tagged
+Inquiry
Cat.#:VCAM1-31199THTag:His
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human VCAM1, His-tagged
+Inquiry
Cat.#:VCAM1-3142HTag:His
Source (Host):HEK293Species:Human
Product nameRecombinant Human VCAM1, His tagged
+Inquiry
Cat.#:VCAM1-6796HTag:His
Source (Host):E. coliSpecies:Human
Product nameRecombinant Human Vascular Cell Adhesion Molecule 1, His-tagged
+Inquiry
Cat.#:VCAM1-68HTag:His
Source (Host):Insect cellsSpecies:Human
Product nameRecombinant Human VCAM1 Protein, His-tagged, Bioactivity Validated
+Inquiry
Cat.#:VCAM1-71HTag:Fc
Source (Host):HEK293Species:Human
Product nameRecombinant Human VCAM1, Fc tagged
+Inquiry
Cat.#:Vcam1-1784MTag:N/A
Source (Host):NS0Species:Mouse
Product nameRecombinant Mouse Vascular Cell Adhesion Molecule 1
+Inquiry
Cat.#:VCAM1-1793MTag:Fc
Source (Host):CHOSpecies:Mouse
Product nameRecombinant Mouse VCAM1, MIgG2a Fc-tagged
+Inquiry
Cat.#:Vcam1-188MTag:His
Source (Host):Insect cellSpecies:Mouse
Product nameRecombinant Mouse Vcam1 protein, His-tagged
+Inquiry
Cat.#:Vcam1-2313MTag:Fc|His
Source (Host):HEK293Species:Mouse
Product nameRecombinant Mouse Vcam1, Fc-His tagged
+Inquiry
Cat.#:Vcam1-2771MTag:His
Source (Host):E. coliSpecies:Mouse
Product nameRecombinant Mouse Vcam1 protein, His-tagged
+Inquiry
Cat.#:Vcam1-2772MTag:His
Source (Host):E. coliSpecies:Mouse
Product nameRecombinant Mouse Vcam1 protein, His-tagged
+Inquiry
Cat.#:Vcam1-4012MTag:His
Source (Host):HEK293Species:Mouse
Product nameRecombinant Mouse Vcam1, His tagged
+Inquiry
Cat.#:Vcam1-4013MTag:His
Source (Host):HEK293Species:Mouse
Product nameRecombinant Mouse Vcam1, His-tagged
+Inquiry
Cat.#:Vcam1-491MTag:Fc Chimera
Source (Host):NSO cellsSpecies:Mouse
Product nameRecombinant Mouse Vcam1, Fc Chimera
+Inquiry
Cat.#:Vcam1-2773RTag:His|T7
Source (Host):E. coliSpecies:Rat
Product nameRecombinant Rat Vcam1 protein, His & T7-tagged
+Inquiry
Cat.#:VCAM1-6505RTag:His
Source (Host):Mammalian CellsSpecies:Rat
Product nameRecombinant Rat VCAM1 Protein
+Inquiry
Cat.#:VCAM1-1534RTag:N/A
Source (Host):HEK293Species:Rhesus monkey
Product nameRecombinant Rhesus Monkey VCAM1 Protein
+Inquiry
Cat.#:VCAM1-1535RTag:hIgG1
Source (Host):HEK293Species:Rhesus monkey
Product nameRecombinant Rhesus Monkey VCAM1 Protein, hIgG1-tagged
+Inquiry
Cat.#:VCAM1-1536RTag:hIgG4
Source (Host):HEK293Species:Rhesus monkey
Product nameRecombinant Rhesus Monkey VCAM1 Protein, hIgG4-tagged
+Inquiry
Cat.#:VCAM1-2775RTag:His|T7
Source (Host):E. coliSpecies:Rhesus monkey
Product nameRecombinant Rhesus monkey VCAM1 protein, His & T7-tagged
+Inquiry
Cat.#:VCAM1-2770DTag:His
Source (Host):E. coliSpecies:Dog
Product nameRecombinant Dog VCAM1 protein, His-tagged
+Inquiry
Cat.#:VCAM1-2774RTag:His|S
Source (Host):E. coliSpecies:Rabbit
Product nameRecombinant Rabbit VCAM1 protein, His & S-tagged
+Inquiry
Cat.#:VCAM1-5452ZTag:His
Source (Host):Mammalian CellsSpecies:Zebrafish
Product nameRecombinant Zebrafish VCAM1
+Inquiry
Cat.#:VCAM1-1695HTag:N/A
Source (Host):NS0Species:Human
Product nameRecombinant Human Vascular Cell Adhesion Molecule 1
+Inquiry
Cat.#:VCAM1-2439HCLTag:N/A
Source (Host):HEK293Species:Human
Product nameRecombinant Human VCAM1 cell lysate
+Inquiry
Cat.#:VCAM1-2094MCLTag:N/A
Source (Host):HEK293Species:Mouse
Product nameRecombinant Mouse VCAM1 cell lysate
+Inquiry
Cat.#:VCAM1-2653MCLTag:N/A
Source (Host):HEK293Species:Mouse
Product nameRecombinant Mouse VCAM1 cell lysate
+Inquiry

Involved Pathway

VCAM1 involved in several pathways and played different roles in them. We selected most pathways VCAM1 participated on our site, such as NF-kappa B signaling pathway, Cell adhesion molecules (CAMs), TNF signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with VCAM1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name
Pathway Related Protein
NF-kappa B signaling pathway
Bcl2a1b; CD40LG; BCL2A1; IL1R1; NFKB1; TLR4; SYK; ICAM1; BIRC2; TNFSF11
Cell adhesion molecules (CAMs)
PVRL1; ICAM3; NADL1.2; MHC2DEB; LRRC4; SELPLG; CD276; OCLN; PVR; NFASCA
TNF signaling pathway
NFKBIA; CREB5; CREB3L3; PIK3CB; FADD; MAP2K7; TAB2; MAP2K4; FAS; RIPK1
Leukocyte transendothelial migration
Itgam&Itgb2; PTPN11; GNAI3; ARHGAP35; ESAM; GNAI2; EZR; ITGA4; RASSF5; RAC1
African trypanosomiasis
HBA1; MYD88; HBB-B2; IL12A; APOA1; IL1B; PLCB4; THOP1; HBB; IL1B2
Malaria
THBS2; HBB; LRP1; Ccl12; COMP; ICAM1; KLRB1; HBA-A2; ITGAL; IFNG
HTLV-I infection
ATM; SMAD2; WNT4; H2-AA; HLA-DQB1; CDC23; PPP3CA; CCND2; CCND1; DLG1

Protein Function

VCAM1 has several biochemical functions, for example, cell adhesion molecule binding, integrin binding, primary amine oxidase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by VCAM1 itself. We selected most functions VCAM1 had, and list some proteins which have the same functions with VCAM1. You can find most of the proteins on our site.
Function
Related Protein
Function cell adhesion molecule binding
Related Protein GRIN2A; CADM3; POSTNB; NLGN2; PKP3; ITGB1; PVRL2L; NLGN3; TIGIT; CD226
Function integrin binding
Related Protein VCAM1; CYR61L1; SYK; ICAM3; WISP1; FCER2; IGF1; ADAM17; FBN1; FBLN1
Function primary amine oxidase activity
Related Protein MAOB; AOC2; AOC3; ABP1; VCAM1; AOC1; MAOA

Interacting Protein

VCAM1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with VCAM1 here. Most of them are supplied by our site. Hope this information will be useful for your research of VCAM1.
ITGB1; ZYX; TLN1; PSMD11

VCAM1 Related Articles

Almeda, D; Wang, BR; et al. Minimizing antibody surface density on liposomes while sustaining cytokine-activated EC targeting. BIOMATERIALS 41:37-44(2015).
Marschall, AL; Single, FN; et al. Functional knock down of VCAM1 in mice mediated by endoplasmatic reticulum retained intrabodies. MABS 6:1394-1401(2014).