| Cat. No. : |
CSF1-167H |
| Description : |
M-CSF (macrophage colony stimulating factor), also known as colony stimulating factor-1 (CSF-1), is a homodimeric glycoprotein the subunits of which are linked by disulfide bonds. Different splice variants of M-CSF have been found which differ at the C-terminus. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
EEVSEYCSHMIGSGH LQSLQRLIDSQME TSCQITFEFV DQEQLKDPV CYLKK AFL LV QD I ME DTMR FRDNTPNAIA IVQLQELSLR LK SCFTK DYEEH DKACV RTF YE TPLQL LE K V KNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCN. |
| Molecular Mass : |
Under reducing conditionsM-CSFmigrates as two bands between 20 and 35 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified M-CSF that has a predicted molecular mass of 18.4 kDa. |
| PI : |
M-CSF separates into a number of isoforms with a pI between 3.5 and 5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified M-CSF that has a predicted pI of 4.8. |
| % Carbohydrate : |
Purified M-CSF consists of 0–50% carbohydrate by weight. |
| Glycosylation : |
M-CSF contains N- and O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilised products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| Activity : |
The ED50of M-CSF is typically 5–15 ng /ml as measured in a cell proliferation assay using the M-CSF dependent mouse monocytic mNFS-60 cell line. |
