Active Recombinant Human IL27/EBI3 protein, His-tagged IL27-881H

Active Recombinant Human IL27/EBI3 protein, His-tagged


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Cat.No. : IL27-881H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human IL27B(Phe 29-Pro 243)/EBI3(Met 1-Lys 229) fused with His tag at C-terminal was expressed in CHO.
Source : CHO
Species : Human
Tag : His
Predicted N Terminal : Arg 21
Form : Lyophilized from sterile PBS, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc, The EC50 of human IL27RA-Fc is 0.2-0.46 μg/ml.2. Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus(VSV). The ED50 for this effect is typically 5-20 ng/mL.
Molecular Mass : The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.
Protein length : IL27(Phe 29-Pro 243)/EBI3(Met 1-Lys 229)
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : IL27 interleukin 27 [ Homo sapiens ]
Official Symbol : IL27
Synonyms : p28; IL30; IL-27; IL27A; IL-27A; IL27p28; IL-27 p28 subunit; IL-27 subunit alpha; IL-27-A; IL27-A; interleukin 30; interleukin-30
Gene ID : 246778
mRNA Refseq : NM_145659
Protein Refseq : NP_663634
MIM : 608273
UniProt ID : Q8NEV9
Chromosome Location : 16p11
Pathway : IL27-mediated signaling events, organism-specific biosystem
Function : cytokine activity; interleukin-27 receptor binding; receptor binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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