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Native Bovine Pyruvate Dehydrogenase (lipoamide) Beta

Cat. No.: PDHB-1860B
Product Overview: Native Bovine PDHBwas expessed in bovine heart.
Description: PDHB, also known aspyruvate dehydrogenase E1 component subunit beta, mitochondrial or PDHE1-B isan enzyme that in humans is encoded by the PDHB gene. The PDHB is anuclear-encoded mitochondrial multienzyme complex that catalyzes the overallconversion of pyruvate to acetyl-CoA and CO2, and provides the primary linkbetween glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex iscomposed of multiple copies of three enzymatic components: pyruvatedehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamidedehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and twobeta subunits. This gene encodes the E1 beta subunit.
Form: Liquid in 50 mMTris-HCl, pH 7.0 + 14 mM β-mercaptoethanol + 1 mM benzamidine +0.1 mM phenylmethanesulfonyl fluoride + 1 mM EDTA + 10% glycerol
Source: Bovine heart
Purity: > 90% asdetermined by SDS-PAGE analysis, subunit apparent Mr"s ~ 74,000 (E2), 53,000(E3), 41,000 (E1a), 48,000 (E3-BP) and 36,000 (E1b). Apparent Mr ~ 8,500,000by sedimentation equilibrium centrifugation.
Concentration: 0.2 mg/ml
Specific Activity: 12-18 µmol of NADHformed per minute per mg of protein with pyruvate as substrate.
Storage: Store in workingaliquots at -80°C. Avoid repeated freeze-thaw cycles.
OfficialSymbol: PDHB
Gene Name: PDHB pyruvate dehydrogenase(lipoamide) beta [ Bos taurus ]
Synonyms: PDHB; pyruvatedehydrogenase (lipoamide) beta; pyruvate dehydrogenase E1 component subunitbeta, mitochondrial; PDHE1-B; EC 1.2.4.1; PDHA; PDHAL; PDHCE1A; PHE1A; PHE1
Gene ID: 613610
mRNA Refseq: NM_001035435
Protein Refseq: NP_001030512
UniProt ID: P11966
Pathway: Butanoatemetabolism; Citrate cycle (TCA cycle); Glycolysis/Gluconeogenesis; Metabolicpathways; Pyruvate metabolism; Pyruvate metabolism and Citric Acid (TCA)cycle; Pyruvate oxidation, pyruvate => acetyl-CoA; Regulation of pyruvatedehydrogenase (PDH) complex; The citric acid (TCA) cycle and respiratoryelectron transport; Valine, leucine and isoleucine biosynthesis; Valine,leucine and isoleucine biosynthesis; pyruvate dehydrogenase complex;superpathway of acetyl-CoA biosynthesis
Function: oxidoreductaseactivity; pyruvate dehydrogenase (acetyl-transferring) activity

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