| Species : |
Mouse |
| Source : |
Mammalian Cells |
| Tag : |
His |
| Protein Length : |
25-184 a.a. |
| Description : |
Grem1 wasidentified in a Xenopus expression-cloning screen as a dorsalizing factorthat can induce a secondary axis. A rat homolog, called Grem1, was identifiedas a cDNA that was downregulated in v-mos transfected cells. Grem1 belongs tothe DAN family of secreted glycoproteins that are BMP antagonists. Othermembers of the family include: cerberus, Dante, PRDC, caronte and DAN. DAN familymembers share a cysteine-rich domain that is structurally related to thecysteine-knot motif found in TGF-β superfamily ligands. In vitro, Grem1 bindsBMP-4 and BMP-2 indicating that it might interfere with BMP signaling. |
| Form : |
Lyophilized from a0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrierprotein. |
| N-terminalSequence : |
Lys25 |
| MolecularWeight : |
19.6 kDa |
| Activity : |
Measuredby its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5mouse chondrogenic cells. The ED50 for this effect is typically 0.4-2 μg/mLin the presence of 75 ng/mL of rhBMP-4. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
23-31kDa, reducing conditions. |
| Purity : |
>90%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
250 μg/mLin sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin |
| Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 monthsfrom date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C understerile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution. |
