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Recombinant Mouse Gremlin 1, His-tagged

Cat. No.: Grem1-5635M
Product Overview: RecombinantMouse Grem1 protein was expressed in Murine myeloma cellline. Lys25-Asp184,with a C-terminal 10-His tag.
Description: Grem1 wasidentified in a Xenopus expression-cloning screen as a dorsalizing factorthat can induce a secondary axis. A rat homolog, called Grem1, was identifiedas a cDNA that was downregulated in v-mos transfected cells. Grem1 belongs tothe DAN family of secreted glycoproteins that are BMP antagonists. Othermembers of the family include: cerberus, Dante, PRDC, caronte and DAN. DAN familymembers share a cysteine-rich domain that is structurally related to thecysteine-knot motif found in TGF-β superfamily ligands. In vitro, Grem1 bindsBMP-4 and BMP-2 indicating that it might interfere with BMP signaling.
Source: Murine myeloma cellline, NS0-derived.
Form: Lyophilized from a0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
N-terminalSequence: Lys25
MolecularWeight: 19.6 kDa
Activity: Measuredby its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5mouse chondrogenic cells. The ED50 for this effect is typically 0.4-2 μg/mLin the presence of 75 ng/mL of rhBMP-4.
Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE: 23-31kDa, reducing conditions.
Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: 250 μg/mLin sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin
Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 monthsfrom date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C understerile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
Gene Name: Grem1 gremlin 1 [ Musmusculus ]
Official Symbol: Grem1
Synonyms: Grem1; gremlin 1;ld; Drm; Cktsf1b1; gremlin-1; down-regulated in v-mos-transformed cells;cysteine knot superfamily 1, BMP antagonist 1; down-regulated inMos-transformed cells protein
Gene ID: 23892
mRNA Refseq: NM_011824
Protein Refseq: NP_035954
UniProt ID: O70326
Chromosome Location: 2
Function: cytokine activity

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