| Cat. No. : |
IL36RN-5660H |
| Description : |
IL36RN isa member of the IL-1 family of proteins. IL-1 family members include IL-1β,IL-1α, IL-1ra, IL-18 and IL-1F5-F10.All family members show a 12 β-strand,β-trefoil configuration, and all family members are believed to have arisenfrom a common ancestral gene that underwent multiple duplications. The human IL-36Ra/IL-1F5gene is in closest proximity to the gene for IL-1ra and is likely arelatively recent duplication of the IL-1ra gene. |
| Source : |
E. coli |
| Form : |
Lyophilizedfrom a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| N-terminalSequence : |
Val2 |
| MolecularWeight : |
17 kDa |
| Activity : |
Measuredby its ability to inhibit IL-36α, IL-36β or IL-36γ-induced IL-8 secretion by humanpreadipocytes. The ED50 for this effect is typically 0.25-1.25 μg/mL in thepresence of 20 ng/mL of recombinant human IL-36β. Measured by its bindingability in a functional ELISA. Immobilized recombinant human IL-36Ra at 1μg/mL (100 μL/well) can bind recombinant human IL-1 Rrp2 Fc Chimera with alinear range of 0.15-5 μg/mL. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
65 kDa,reducing conditions. |
| Purity : |
>90%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
100 μg/mLin sterile PBS |
| Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution. |
