Recombinant Human NAT1, His-tagged

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Recombinant Human NAT1, His-tagged

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Cat. No. : NAT1-3573H
Product Overview : Recombinant human NAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-290 aa).
Description : NAT1 belongs to the arylamine N-acetyltransferase family. It catalyzes N- or O-acetylation of heterocyclic and arylamine substrates in the detoxification of a wide array of drugs. Certain alleles causing high levels of N-acetyltransferase activity have been associated with colon and urinary bladder cancers, as NAT1 also bioactivate several known carcinogens. This enzyme helps metabolize drugs and other xenobiotics, and participates in the detoxification of a plethora of hydrazine and arylamine drugs.
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Molecular Weight : 36.1 kDa (310aa), confirmed by MALDI-TOF
Purity : > 85 % by SDS - PAGE
Concentration : 0.5 mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MDIEAYLERI GYKKSRNKLD LETLTDILQH QIRAVPFENL NIHCGDAMDL GLEAIFDQVV RRNRGGWCLQ VNHLLYWALT TIGFETTMLG GYVYSTPAKK YSTGMIHLLL QVTIDGRNYI VDAGFGRSYQ MWQPLELISG KDQPQVPCIF RLTEENGFWY LDQIRREQYI PNEEFLHSDL LEDSKYRKIY SFTLKPRTIE DFESMNTYLQ TSPASVFTSK SFCSLQTPDG VHCLVGFTLT HRRFNYKDNT DLIEFKTLSE EEIEKVLKNI FNISLQRKLV PKHGDRFFTI
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : NAT1 N-acetyltransferase 1 (arylamine N-acetyltransferase) [ Homo sapiens ]
Official Symbol : NAT1
Synonyms : NAT1; AAC1; MNAT; NATI; NAT-1; arylamine N-acetyltransferase 1; OTTHUMP00000122488; OTTHUMP00000224577; OTTHUMP00000224578; OTTHUMP00000224579; arylamide acetylase 1; N-acetyltransferase type 1; monomorphic arylamine N-acetyltransferase; EC 2.3.1.5; N-acetyltransferase 1 (arylamine N-acetyltransferase); Arylamide acetylase 1; Monomorphic arylamine N-acetyltransferase
Gene ID : 9
mRNA Refseq : NM_000662
Protein Refseq : NP_000653
MIM : 108345
UniProt ID : P18440
Chromosome Location : 8p22
Function : acetyltransferase activity; arylamine N-acetyltransferase activity; transferase activity

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