Active Recombinant Mouse Chemokine (C-C motif) Ligand 7

• Cat.No.: Ccl7-287M
• Product Overview: Recombinant mouse MCP-3 is produced in E.Coli,and is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Mouse MCP-3, also known as CCL7 and SCYA7 is produced in a variety of tumor cells and has been shown to induce chemotaxis in monocytes, basophils, NK cells and dendrocytes.
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: Mouse MCP-3 is fully biologically active when compared to standard. The specific activity as determined by the ability of Mouse MCP-3 to chemoattract Balb/C mouse spleen MNCs at 10-100ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Amino acid sequence: QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
• Pathways: Chemokine signaling pathway; Cytokine-cytokine receptor interaction

Gene Information

• Gene Name: Ccl7 chemokine (C-C motif) ligand 7 [ Mus musculus ]
• Official Symbol: Ccl7
• Synonyms: Ccl7; chemokine (C-C motif) ligand 7; fic; marc; mcp3; MCP-3; Scya7; C-C motif chemokine 7; OTTMUSP00000000787; RANTES/sis homolog; intercrine/chemokine MARC; small inducible cytokine A7; small-inducible cytokine A7; monocyte chemotactic protein 3; monocyte chemoattractant protein 3; MCP3; Monocyte chemoattractant protein 3; NC28; SCYA6; SCYA7; MGC138463; FIC; MGC138465; MARC; small inducible cytokine A7 (monocyte chemotactic protein 3)
• Gene ID: 20306
• mRNA Refseq: NM_013654
• Protein Refseq: NP_038682
• UniProt ID: Q03366
• Chromosome Location: 11 C; 11 46.5 cM
• Function: CCR2 chemokine receptor binding; chemokine activity; cytokine activity; heparin binding