Recombinant Bovine Enterokinase


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Recombinant Bovine Enterokinase

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Cat. No. : PRSS7-02B
Product Overview : Recombinant Bovine Enterokinase produced in Pichia. Pastoris is a single glycosylated polypeptide chain containing 235 amino acids and having a molecular mass of approximately 43.0 kDa.
Description : Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It is the physiological activator of pancreatic trypsinogen. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.
Unit Definition : One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 25°C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.
Formulation : 50 mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.
Physical Appearance : Sterile liquid
Endotoxin : Less than 1EU/mg of rbEK as determined by LAL method.
Storage : One year when stored at -20°C. Avoid repeated freeze/thaw cycles.
Enzyme Commission Number : EC3.4.21.9
Reaction : The reactioncatalysedby Enteropeptidase: trypsinogen → trypsin + octapeptide Enteropeptidase cleaves after Lysine if the Lys is preceded by four Asp and not followed by a Pro.
Crystal structure of Enteropeptidase with an inhibitor protease, serine, 7 (human) :

Gene Name : protease, serine, 7 (enterokinase)
Synonyms : PRSS7; EC; ENTK; Enterokinase; MGC133046; enterokinase; enteropeptidase; proenterokinase; Enteropeptidase precursor; Serine protease 7; human enterokinase; EC; protease, serine, 7; serine protease (enterokinase); protease, serine, 7 (enterokinase)
Gene ID : 282009
mRNA Refseq : NM_174439
Protein Refseq : NP_776864
UniProt ID : P98072
Chromosome Location : 1
Function : peptidase activity;scavenger receptor activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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