Active Recombinant Canine IL17A protein(Gly26-Ala155)
Cat.No. : | IL17A-168C |
Product Overview : | Recombinant Canine IL17A (NP_001159350.1)(Gly26-Ala155) was expressed in E. coli with a N-terminal Met. |
- Specification
- Gene Information
- Related Products
Source : | E. coli |
Species : | Canine |
Tag : | N/A |
Protein length : | Gly26-Ala155 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized Canine IL17A at 10 μg/ml (100 μl/well) can bind human IL17Ra, The EC50 of human IL17Ra is 6.0-15.0 ng/ml. 2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The ED50 for this effect is typically 1-5 ng/mL. |
Molecular Mass : | The recombinant canine IL17A comprises 131 amino acids and has a predicted molecular mass of 15.2 kDa. The apparent molecular mass of the protein is approximately 15 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
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◆ Lysates | ||
IL17A-001HCL | Recombinant Human IL17A cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAnimal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.
Inducing IL17A expression in T cells involves culturing them with specific cytokines.
Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.
Neutralizing antibodies block IL17A's effects in cell cultures or animal models.
Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.
IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.
Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.
Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.
Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.
Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.
Customer Reviews (3)
Write a reviewGood for receptor studies.
Effective in Th17 differentiation.
High bioactivity in various tissue types.
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