"FGF23" Related Products

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Recombinant Human FGF23 protein

Cat.No.: FGF23-523H
Product Overview: Recombinant Human FGF23 was expressed in E. coli.
Source: E. coli
Species: Human
Form: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10^3 IU/mg in the presence of 0.3 μg/ml of rMuKlotho and 10 μg/ml of heparin.
Molecular Mass: Approximately 25.3 kDa, a single non-glycosylated polypeptide chain containing 227 amino acids.
Protein length: 227 amino acids
AA Sequence: YPNASPLLGS SWGGLIHLYT ATARNSYHLQ IHKNGHVDGA PHQTIYSALM IRSEDAGFVV ITGVMSRRYL CMDFRGNIFG SHYFDPENCR FQHQTLENGY DVYHSPQYHF LVSLGRAKRA FLPGMNPPPY SQFLSRRNEI PLIHFNTPIP RRHTRSAEDD SERDPLNVLK PRARMTPAPA SCSQELPSAE DNSPMASDPL GVVRGGRVNT HAGGTGPEGC RPFAKFI
Endotoxin: Less than 1 EU/µg of rHuFGF-23 as determined by LAL method.
Purity: > 95 % by SDS-PAGE and HPLC analyses.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name: FGF23 fibroblast growth factor 23 [ Homo sapiens ]
Official Symbol: FGF23
Synonyms: FGF23; fibroblast growth factor 23;
Gene ID: 8074
mRNA Refseq: NM_020638
Protein Refseq: NP_065689
MIM:
UniProt ID: Q9GZV9
Chromosome Location: 12p13.3
Pathway: ARMS-mediated activation, organism-specific biosystem; Axon guidance, organism-specific biosystem; Diseases of signal transduction, organism-specific biosystem
Function: 1-phosphatidylinositol-3-kinase activity; growth factor activity; protein tyrosine kinase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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