Recombinant Mouse IL12A & IL12B cell lysate
Cat.No. : | IL12A & IL12B-1782MCL |
Product Overview : | Mouse IL12A & IL12B Heterodimer derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Il12a interleukin 12a [ Mus musculus ]; Il12b interleukin 12b [ Mus musculus ] |
Official Symbol : | Il12a; Il12b |
Synonyms : | p35; Ll12a; Il-12a; IL-12p35; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p40; Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit |
Gene ID : | 16159; 16160 |
mRNA Refseq : | NM_001159424; NM_008352 |
Protein Refseq : | NP_001152896; NP_032378 |
UniProt ID : | P43431; P43432 |
Chromosome Location : | 3 E1; 3 31.92 cM; 11 A5-B2; 11 25.94 cM |
Pathway : | African trypanosomiasis, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; African trypanosomiasis, organism-specific biosystem; Amoebiasis, conserved biosystem |
Function : | contributes_to cytokine activity; interleukin-12 receptor binding; contributes_to cytokine activity; contributes_to growth factor activity |
Products Types
◆ Recombinant Protein | ||
IL12A-176H | Active Recombinant Human IL12A Protein (Arg23-Ser219), C-His tagged, Animal-free, Carrier-free | +Inquiry |
Il12a-086M | Active Recombinant Mouse Il12a Protein | +Inquiry |
Il12a-140M | Recombinant Active Mouse IL12A Protein, His-tagged(C-ter) | +Inquiry |
IL12A-4327HG | Active Recombinant Human IL12A protein | +Inquiry |
IL12A-2680R | Recombinant Rat IL12A Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
IL12A-001CCL | Recombinant Cynomolgus IL12A cell lysate | +Inquiry |
IL12A-2435HCL | Recombinant Human IL12A cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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