- Human(5)
Recombinant Human AP3B1 293 Cell Lysate
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Cat.No. : | AP3B1-8810HCL |
Description : | Antigen standard for adaptor-related protein complex 3, beta 1 subunit (AP3B1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AP3B1 adaptor-related protein complex 3, beta 1 subunit [ Homo sapiens ] |
Official Symbol : | AP3B1 |
Synonyms : | AP3B1; adaptor-related protein complex 3, beta 1 subunit; AP-3 complex subunit beta-1; ADTB3A; HPS2; beta3A-adaptin; beta-3A-adaptin; AP-3 complex beta-3A subunit; adaptor protein complex AP-3 subunit beta-1; adapter-related protein complex 3 subunit beta-1; clathrin assembly protein complex 3 beta-1 large chain; PE; HPS; ADTB3; |
Gene ID : | 8546 |
mRNA Refseq : | NM_003664 |
Protein Refseq : | NP_003655 |
MIM : | 603401 |
UniProt ID : | O00203 |
Chromosome Location : | 5q14.1 |
Pathway : | Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Membrane Trafficking, organism-specific biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem; |
Function : | binding; protein phosphatase binding; protein transporter activity; |
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AP2M1 AP3S2 AP1S2 AP2S1 AP3B1 AP4B1 AP4M1 AP2B1 AP1G1 AP1B1 AP1S1 AP2A1 AP2A2 AP1M1 AP1M2 AP3D1 AP3M2 AP3M1 AP3S1 AP3B2 AP1AR AP4E1 AP4S1 AP5B1 AP5M1 AP5S1 Ap1g2 AP1S3 AP5Z1; AP-5 complex subunit zeta-1; zeta5; adapter-related protein complex 5 zeta subunit
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